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Menadione-induced oxidative stress in bovine heart microvascular endothelial cells.
- Source :
-
Microcirculation (New York, N.Y. : 1994) [Microcirculation] 1996 Mar; Vol. 3 (1), pp. 39-47. - Publication Year :
- 1996
-
Abstract
- Objective: Oxidative stress from increased production of reactive oxygen species or decreased efficiency of inhibitory and scavenger systems may contribute to vascular injury. In this study, we developed an in vitro model of vascular injury by menadione-induced oxidative stress in bovine heart microvascular endothelial cells.<br />Methods: Oxidative stress was induced by exposure to menadione. Superoxide, hydrogen peroxide and hydroxyl radical formation was measured by superoxide dismutase-inhibitable cytochrome c reduction, the dichlorofluorescin technique and the salicylate method, respectively. Electron paramagnetic-spin resonance spectroscopy employing 5-5'-dimethyl-l-pyrroline-N-oxide for superoxide trapping was used. Endothelial cell cytotoxicity was assessed by lactate dehydrogenase release.<br />Results: Superoxide and hydroxyl radical were produced in a time- and concentration-dependent fashion. Fluorescence in the presence of dichlorofluorescin confirmed hydrogen peroxide formation. Endothelial cell cytotoxicity became evident after 5 h of menadione treatment at concentrations of 100 microM. 3-Aminobenzamide, a poly(ADP-ribose)polymerase inhibitor, and dimethylthiourea, a hydrogen peroxide and hydroxyl radical scavenger, decreased menadione cytotoxicity, whereas deferoxamine, an inhibitor of hydroxyl radical formation, did not.<br />Conclusions: The results suggest that menadione toxicity is mediated by poly(ADP-ribose)polymerase activation via hydrogen peroxide formation and that menadione-treated bovine heart microvessel endothelial cells provide a suitable in vitro model to study oxidative stress in endothelial cells.
- Subjects :
- Animals
Cattle
Cell Death drug effects
Cell Membrane drug effects
Endothelium, Vascular cytology
Enzyme Activation
Free Radical Scavengers pharmacology
Hydrogen Peroxide metabolism
Hydroxyl Radical
Microcirculation drug effects
Poly(ADP-ribose) Polymerases agonists
Superoxides metabolism
Coronary Circulation drug effects
Endothelium, Vascular drug effects
Oxidative Stress drug effects
Reactive Oxygen Species metabolism
Vitamin K pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 1073-9688
- Volume :
- 3
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Microcirculation (New York, N.Y. : 1994)
- Publication Type :
- Academic Journal
- Accession number :
- 8846270
- Full Text :
- https://doi.org/10.3109/10739689609146781