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Characterization of structural proteins of Lelystad virus.
- Source :
-
Advances in experimental medicine and biology [Adv Exp Med Biol] 1995; Vol. 380, pp. 271-6. - Publication Year :
- 1995
-
Abstract
- The genome of Lelystad virus (LV), a positive-strand RNA virus, is 15 kb in length and contains 8 open reading frames that encode putative viral proteins. Synthetic polypeptides of 15 to 17 amino acids were selected from the amino acid sequences of ORFs 2 to 7 and anti-peptide sera were raised in rabbits. Using these anti-peptide sera and porcine anti-LV serum, we identified three structural proteins and assigned their corresponding genes. Virions were found to contain a nucleocapsid protein of 15 kDa (N), an unglycosylated membrane protein of 18 kDa (M), and a glycosylated membrane protein of 25 kDa (E). The N protein is encoded by ORF7, the M protein is encoded by ORF6, and the E protein is encoded by ORF5.
- Subjects :
- Amino Acid Sequence
Animals
Antibodies
Antigen-Antibody Reactions
Blotting, Western
Capsid biosynthesis
Cell Line
Cells, Cultured
Macrophages, Alveolar virology
Molecular Sequence Data
Open Reading Frames
Rabbits immunology
Swine
Viral Core Proteins biosynthesis
Viral Matrix Proteins biosynthesis
Viral Structural Proteins isolation & purification
Virion genetics
Virion metabolism
RNA Viruses genetics
RNA Viruses metabolism
Viral Structural Proteins biosynthesis
Viral Structural Proteins chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0065-2598
- Volume :
- 380
- Database :
- MEDLINE
- Journal :
- Advances in experimental medicine and biology
- Publication Type :
- Academic Journal
- Accession number :
- 8830491
- Full Text :
- https://doi.org/10.1007/978-1-4615-1899-0_43