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Local and systemic factors in periodontal disease increase matrix-degrading enzyme activities in rat gingiva: effect of micocycline therapy.
- Source :
-
Research communications in molecular pathology and pharmacology [Res Commun Mol Pathol Pharmacol] 1996 Mar; Vol. 91 (3), pp. 303-18. - Publication Year :
- 1996
-
Abstract
- We previously reported that both local and systemic factors relevant to the pathogenesis of periodontal disease can increase gingival collagenase activity in rats. Since the degradation of extracellular matrix is an essential feature of periodontal disease and this tissue breakdown requires multiple enzyme interactions, the current study was carried out to determine the effects of bacterial endotoxin (LPS) (a local factor) and diabetes (a systemic factor) on a panel of matrix-degrading enzymes (collagenase, gelatinase, elastase, and beta-glucuronidase) in the gingiva of rats. In addition, the effects of therapy with a semisynthetic tetracycline (minocycline) were investigated. Ten male, Sprague-Dawley rats were made diabetic by IV injection of streptozotocin. Four of the ten rats then received minocycline (10 mg/day) by oral gavage on a daily basis for 3 weeks. Nineteen nondiabetic rats served as controls and 9 of them received 10 microliters of E. coli LPS (10 mg/ml) by injection into the labial gingiva every other day during the last week of the study. The other 10 nondiabetic rats were sham injected with saline into the gingiva. At the end of the 3 week experimental period, gingival tissue and skin were dissected from each rat and extracted for enzyme analysis. Our results showed that diabetes markedly increased the four matrix-degrading enzyme activities in both gingiva and skin. In contrast, local LPS injection increased these enzyme activities in the gingiva alone. Systemic therapy with minocycline completely ameliorated these elevated enzyme levels in diabetic rats in both gingiva and skin. Minocycline added in vitro to the enzyme assay systems containing skin extract from diabetic rats also inhibited collagenase and gelatinase activities, but no inhibition was observed for elastase and beta-glucuronidase activities, indicating that the MMPs and other enzymes were inhibited by minocycline, during diabetes, by indirect and indirect mechanisms, respectively.
- Subjects :
- Alveolar Bone Loss drug therapy
Alveolar Bone Loss enzymology
Alveolar Bone Loss pathology
Animals
Anti-Bacterial Agents administration & dosage
Blood Glucose metabolism
Body Weight drug effects
Collagen metabolism
Diabetes Mellitus, Experimental blood
Diabetes Mellitus, Experimental complications
Diabetes Mellitus, Experimental enzymology
Extracellular Matrix drug effects
Extracellular Matrix enzymology
Gingiva pathology
Injections
Lipopolysaccharides pharmacology
Male
Minocycline administration & dosage
Periodontal Diseases pathology
Rats
Rats, Sprague-Dawley
Skin drug effects
Skin enzymology
Skin metabolism
Anti-Bacterial Agents therapeutic use
Extracellular Matrix metabolism
Gingiva enzymology
Minocycline therapeutic use
Periodontal Diseases drug therapy
Periodontal Diseases enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 1078-0297
- Volume :
- 91
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Research communications in molecular pathology and pharmacology
- Publication Type :
- Academic Journal
- Accession number :
- 8829770