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Analysis of the DNA sequence of a 15,500 bp fragment near the left telomere of chromosome XV from Saccharomyces cerevisiae reveals a putative sugar transporter, a carboxypeptidase homologue and two new open reading frames.
- Source :
-
Yeast (Chichester, England) [Yeast] 1996 Jun 15; Vol. 12 (7), pp. 709-14. - Publication Year :
- 1996
-
Abstract
- We report the sequence of a 15.5 kb DNA segment located near the left telomere of chromosome XV of Saccharomyces cerevisiae. The sequence contains nine open reading frames (ORFs) longer than 300 bp. Three of them are internal to other ones. One corresponds to the gene LGT3 that encodes a putative sugar transporter. Three adjacent ORFs were separated by two stop codons in frame. These ORFs presented homology with the gene CPS1 that encodes carboxypeptidase S. The stop codons were not found in the same sequence derived from another yeast strain. Two other ORFs without significant homology in databases were also found. One of them, O0420, is very rich in serine and threonine and presents a series of repeated or similar amino acid stretches along the sequence.
- Subjects :
- Amino Acid Sequence
Base Sequence
Carboxypeptidases genetics
Codon, Terminator genetics
DNA Primers genetics
Molecular Sequence Data
Monosaccharide Transport Proteins genetics
Open Reading Frames
Repetitive Sequences, Nucleic Acid
Restriction Mapping
Sequence Homology, Amino Acid
Telomere genetics
Chromosomes, Fungal genetics
DNA, Fungal genetics
Saccharomyces cerevisiae genetics
Saccharomyces cerevisiae Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0749-503X
- Volume :
- 12
- Issue :
- 7
- Database :
- MEDLINE
- Journal :
- Yeast (Chichester, England)
- Publication Type :
- Academic Journal
- Accession number :
- 8810044
- Full Text :
- https://doi.org/10.1002/(SICI)1097-0061(19960615)12:7%3C709::AID-YEA957%3E3.0.CO;2-1