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Menstrual breakdown of human endometrium can be mimicked in vitro and is selectively and reversibly blocked by inhibitors of matrix metalloproteinases.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1996 Aug 20; Vol. 93 (17), pp. 9120-5. - Publication Year :
- 1996
-
Abstract
- The mechanisms underlying the menstrual lysis leading to shedding of the human endometrium and its accompanying bleeding are still largely unknown. In particular, whether breakdown of the endometrial fibrillar extra-cellular matrix that precedes bleeding depends on aspartic-, cysteine-, serine-, or metalloproteinases remains unclear. In the present study, menstrual regression of the human endometrium was mimicked in organ culture. Whereas sex steroids could preserve tissue integrity only in nonperimenstrual explants, matrix breakdown upon sex steroid deprivation was completely and reversibly inhibited at all stages of the menstrual cycle by specific inhibitors of matrix metalloproteinases, but not by inhibitors of the other classes of proteinases. Matrix metalloproteinases are thus identified as the key class of proteinases involved in the initiation of menstruation.
- Subjects :
- Collagen metabolism
Endometrium enzymology
Enzyme Activation
Extracellular Matrix ultrastructure
Female
Humans
Immunohistochemistry
Kinetics
Metalloendopeptidases antagonists & inhibitors
Organ Culture Techniques
Protease Inhibitors pharmacology
Silver Staining
Endometrium physiology
Extracellular Matrix metabolism
Menstruation physiology
Metalloendopeptidases metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 93
- Issue :
- 17
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 8799164
- Full Text :
- https://doi.org/10.1073/pnas.93.17.9120