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Hypoxia-induced necrotizing enterocolitis in the immature rat: the role of lipid peroxidation and management by vitamin E.
- Source :
-
Journal of pediatric surgery [J Pediatr Surg] 1995 Oct; Vol. 30 (10), pp. 1416-9. - Publication Year :
- 1995
-
Abstract
- The authors developed an experimental model of necrotizing enterocolitis (NEC) by hypoxia-reoxygenation, and determined the content of malondialdehyde levels as an index of lipid peroxidation, related with a free-radical reaction in the gastrointestinal tract of newborn rats. They also investigated the role of vitamin E, an antioxidant, in this free-radical injury. The study was performed on 1-day-old rats. The 30 rat pups were divided into three groups. Hypoxia was induced by placing the pups in a 100% carbon dioxide chamber for 5 minutes. The pups were reoxygenated with 100% oxygen for 5 minutes. Group 1 (n = 10) was subjected to hypoxia-reoxygenation and killed 3 days after hypoxia. Group 2 (n = 10) was subjected to hypoxia-reoxygenation and treated with vitamin E (30 IU/kg/d intraperitoneally) for the next 3 days, and killed. Group 3 (n = 10) rats served as controls. The histopathology of the intestinal lesions in group 1 animals was characteristic of ischemic injury and ranged from superficial epithelial damage with villous shortening to transmural necrosis. In the vitamin E-treated animals these lesions were milder. The malondialdehyde levels of group 1 were significantly higher than those of the other two groups (P < .001). This study shows that oxidant-mediated lipid peroxidation injury plays a central role in mediating hypoxia-induced intestinal necrosis and suggests that vitamin E may play a therapeutic role in NEC.
- Subjects :
- Animals
Animals, Newborn
Disease Models, Animal
Enterocolitis, Pseudomembranous etiology
Free Radicals
Malondialdehyde metabolism
Rats
Rats, Wistar
Enterocolitis, Pseudomembranous drug therapy
Enterocolitis, Pseudomembranous metabolism
Hypoxia complications
Lipid Peroxidation
Vitamin E therapeutic use
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3468
- Volume :
- 30
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of pediatric surgery
- Publication Type :
- Academic Journal
- Accession number :
- 8786477
- Full Text :
- https://doi.org/10.1016/0022-3468(95)90395-x