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DNA strand scission by benzo[a]pyrene diol epoxides.

Authors :
Gamper HB
Tung AS
Straub K
Bartholomew JC
Calvin M
Source :
Science (New York, N.Y.) [Science] 1977 Aug 12; Vol. 197 (4304), pp. 671-4.
Publication Year :
1977

Abstract

Syn-and anti-benzo[a]pyrene diol epoxides elicit a concentration-dependent nicking of superhelical Col E1 DNA in an in vitro reaction monitored by agarose gel electrophoresis and electron microscopy. This strand scission represents less than 1 percent of the DNA modification by diol epoxide. Kinetic analysis implicates the formation of unstable phosphotriesters, hydrolysis of which nick the DNA.

Subjects

Subjects :
Ethers, Cyclic
Hydrolysis
Kinetics
Nucleic Acid Conformation drug effects
Plasmids
RNA, Viral
Structure-Activity Relationship
Benzopyrenes
DNA, Bacterial
DNA, Circular

Details

Language :
English
ISSN :
0036-8075
Volume :
197
Issue :
4304
Database :
MEDLINE
Journal :
Science (New York, N.Y.)
Publication Type :
Academic Journal
Accession number :
877583
Full Text :
https://doi.org/10.1126/science.877583
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