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[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].
[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].
- Source :
-
Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 1996 Feb; Vol. 76 (2), pp. 96-9. - Publication Year :
- 1996
-
Abstract
- Objective: To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells.<br />Methods: The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate.<br />Results: Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining.<br />Conclusion: In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.
- Subjects :
- Adenoma pathology
Humans
Immunoglobulin Fab Fragments immunology
Tumor Cells, Cultured
Antibodies, Bispecific immunology
Antibodies, Monoclonal immunology
Carcinoma, Hepatocellular pathology
Cytotoxicity, Immunologic
Liver Neoplasms pathology
Lung Neoplasms pathology
Macrophages immunology
Monocytes immunology
Subjects
Details
- Language :
- Chinese
- ISSN :
- 0376-2491
- Volume :
- 76
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Zhonghua yi xue za zhi
- Publication Type :
- Academic Journal
- Accession number :
- 8758438