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[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].

[Anti-tumor effects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells].

Authors :
Wang Q
Chen Z
Wang W
Source :
Zhonghua yi xue za zhi [Zhonghua Yi Xue Za Zhi] 1996 Feb; Vol. 76 (2), pp. 96-9.
Publication Year :
1996

Abstract

Objective: To study anti-tumor affects of bispecific monoclonal antibody targeted human monocytes-macrophages on hepatoma cells.<br />Methods: The fragment of monoclonal antibody HAb18F(ab')2 which has high affinity to hepatoma cells and the fragment of monoclonal antibody antimonocytes/macrophages MAb7F(ab')2 were cross-linked with N-succinimidyl 3-(2-pyridl-dithil) propionate.<br />Results: Heteroaggregated monoclonal antibodies HAb18F(ab')2-MAb7F(ab')2 could bind to hepatoma cells as well as monocytes-macrophages. They were purified with FPLC Sephacryl S-200. The bispecific character was identified with Indirect Immunofluorescent Staining.<br />Conclusion: In vitro cytotoxicity assay showed that HAb18F(ab')2-MAb7F(ab')2 could significantly enhance the lysis of human hepatoma cells by human monocytes-macrophages.

Details

Language :
Chinese
ISSN :
0376-2491
Volume :
76
Issue :
2
Database :
MEDLINE
Journal :
Zhonghua yi xue za zhi
Publication Type :
Academic Journal
Accession number :
8758438