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Cultured leptomeningeal cells secrete cerebrospinal fluid proteins.
- Source :
-
Journal of neurochemistry [J Neurochem] 1996 Sep; Vol. 67 (3), pp. 964-71. - Publication Year :
- 1996
-
Abstract
- To extrapolate the function of the leptomeninges, we examined the profile of the proteins secreted from the cultured leptomeningeal cells prepared from 1-2-day-old rats. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis of the medium conditioned with the cultured cells, 20-25 differentially distinctive protein bands were noted. Through several chromatographic procedures (Sephadex G-75, Mono Q, and 7C8-300), altogether 18 proteins were purified to homogeneity, and the partial amino acid sequence of each protein was determined. Homology search revealed that the major proteins included prostaglandin-D-synthase or beta-trace protein, insulin-like growth factor (IGF)-II, IGF-binding protein-2, apolipoprotein E, beta 2-microglobulin, cystatin C, transferrin, peptidyl-prolyl cis-trans isomerase or cyclophilin C, secreted protein acidic and rich in cysteine, ubiquitin, lysozyme C, extracellular superoxide dismutase, and collagen alpha-1 (III). Most of these proteins are known to be the major brain-derived protein constituents of CSF and are thought to play important roles in certain biological events in the brain. Considering the morphological features, the present findings suggest the importance of the leptomeninges as an origin of such proteins in CSF.
- Subjects :
- Amino Acid Sequence
Animals
Apolipoproteins E cerebrospinal fluid
Apolipoproteins E isolation & purification
Apolipoproteins E metabolism
Arachnoid cytology
Arachnoid metabolism
Cells, Cultured metabolism
Cerebrospinal Fluid chemistry
Cerebrospinal Fluid Proteins isolation & purification
Chromatography
Culture Media, Conditioned
Cystatin C
Cystatins cerebrospinal fluid
Cystatins isolation & purification
Cystatins metabolism
Cysteine Proteinase Inhibitors cerebrospinal fluid
Cysteine Proteinase Inhibitors isolation & purification
Cysteine Proteinase Inhibitors metabolism
Dextrans
Electrophoresis, Polyacrylamide Gel
Gels
Insulin-Like Growth Factor II cerebrospinal fluid
Insulin-Like Growth Factor II isolation & purification
Insulin-Like Growth Factor II metabolism
Meninges metabolism
Molecular Sequence Data
Osteonectin cerebrospinal fluid
Osteonectin isolation & purification
Osteonectin metabolism
Pia Mater cytology
Pia Mater metabolism
Rats
Transferrin cerebrospinal fluid
Transferrin isolation & purification
Transferrin metabolism
beta 2-Microglobulin cerebrospinal fluid
beta 2-Microglobulin isolation & purification
beta 2-Microglobulin metabolism
Cerebrospinal Fluid Proteins metabolism
Meninges cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-3042
- Volume :
- 67
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of neurochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8752101
- Full Text :
- https://doi.org/10.1046/j.1471-4159.1996.67030964.x