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Alcohol inhibits epidermal growth factor-stimulated progesterone secretion from human granulosa cells.
- Source :
-
Alcoholism, clinical and experimental research [Alcohol Clin Exp Res] 1995 Dec; Vol. 19 (6), pp. 1382-8. - Publication Year :
- 1995
-
Abstract
- In this study, luteinized human granulosa cells (GC) obtained during in vitro fertilization procedures were used as a model system to evaluate the effects of ethanol (EtOH), a well-known reproductive toxin, on epidermal growth factor (EGF) and gonadotropin-stimulated steroidogenesis. Our results demonstrate that the basal progesterone (P4) and estradiol (E2) secretion by human GC in vitro was dependent on the ovarian stimulation protocol. EGF significantly enhanced P4, but not E2, secretion in human GC from clomiphene citrate (CC), human menopausal gonadotropin (hMG), and hMG/gonadotropin-releasing hormone agonist (GnRH-a)-treated patients. The effects of EGF plus luteinizing hormone (LH) were additive in cells from the CC group, but less than additive in hMG and hMG/GnRH-a groups. EtOH at 20 mM or more inhibited EGF stimulated P4 secretion in human GC from all three patient groups. EtOH inhibited P4 secretion stimulated by EGF and LH cotreatment in the CC and hMG/GnRH-a groups, but not in human GC from the hMG-treated patients. These results suggest that basal and EGF or LH-stimulated P4 secretion by human GC, as well as the effects of EtOH, are profoundly influenced by the follicle's hormonal milieu.
- Subjects :
- Cells, Cultured
Dose-Response Relationship, Drug
Epidermal Growth Factor pharmacology
Estradiol metabolism
Female
Fertilization in Vitro
Granulosa Cells metabolism
Humans
Ovulation Induction
Epidermal Growth Factor antagonists & inhibitors
Ethanol pharmacology
Granulosa Cells drug effects
Progesterone metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0145-6008
- Volume :
- 19
- Issue :
- 6
- Database :
- MEDLINE
- Journal :
- Alcoholism, clinical and experimental research
- Publication Type :
- Academic Journal
- Accession number :
- 8749799
- Full Text :
- https://doi.org/10.1111/j.1530-0277.1995.tb00996.x