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Basic isoforms of Par o 1, the major allergen of Parietaria officinalis pollen.
- Source :
-
Allergy [Allergy] 1995 Nov; Vol. 50 (11), pp. 899-904. - Publication Year :
- 1995
-
Abstract
- We describe a group of basic isoforms of Par o 1 (cumulatively referred to as Par o 1b), purified by anion-exchange chromatography. The allergenic activity of Par o 1b was compared with that of the acidic isoform (Par o 1a) by RAST inhibition. Par o 1b showed a cathodic mobility in crossed immunoelectrophoresis. It was found to be homogeneous in SDS-PAGE and SE-HPLC (14.5 kDa), and heterogeneous in PAG-IEF, yielding five IgE-binding bands with pI ranging between 7.9 and 9.6 PAG-IEF individual components were isolated by cation-exchange HPLC. The N-terminal amino acid sequence of the main component (pI 8.8) was determined and found to be similar to that of Par o 1a.
- Subjects :
- Allergens isolation & purification
Amino Acid Sequence
Chromatography, High Pressure Liquid
Chromatography, Ion Exchange
Electrophoresis, Polyacrylamide Gel
Immunoelectrophoresis
Isoelectric Point
Molecular Sequence Data
Molecular Weight
Plant Proteins chemistry
Radioallergosorbent Test
Allergens analysis
Pollen chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 0105-4538
- Volume :
- 50
- Issue :
- 11
- Database :
- MEDLINE
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 8748722
- Full Text :
- https://doi.org/10.1111/j.1398-9995.1995.tb02496.x