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In vitro chick pre-cardiac explant tissue differentiation during spaceflight on SpaceHab-02.
- Source :
-
Acta anatomica [Acta Anat (Basel)] 1995; Vol. 154 (3), pp. 169-80. - Publication Year :
- 1995
-
Abstract
- Chick precardiac tissue explants were cultured on the 8-day mission of STS-60, space shuttle Discovery. Development of in vitro cultures of precardiac chick tissue from embryo stages 5 though 8 (H-H) were initiated during orbit and were terminated after approximately fifteen hours of 37 degree C culture. Transmission electron microscopy and tritiated thymidine studies were performed postflight. No significant differences in cell proliferation were observed between flight and ground controls. Electron-microscopic studies revealed stage 8 explants were capable of differentiation during flight in a pattern which matched ground control tissues. As anticipated, stage 7 explant tissues had differentiated to a lesser extent compared to stage 8 tissues. Interestingly, stage 7 precardiac explant flight tissue differentiation was less than ground control tissue. This difference in differentiation between flight and ground cultures was enhanced in stage 6 tissues, as high levels of myofibril organization were only seen in ground controls. Other cellular components such as Golgi apparatus, junctional complexes, and mitochondria were present and appeared normal and healthy.
Details
- Language :
- English
- ISSN :
- 0001-5180
- Volume :
- 154
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Acta anatomica
- Publication Type :
- Academic Journal
- Accession number :
- 8739762
- Full Text :
- https://doi.org/10.1159/000147766