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Manganese and 1-methyl-4-(2'-ethylpheny1)-1,2,3,6-tetrahydropyridine induce apoptosis in PC12 cells.
- Source :
-
Neuroscience letters [Neurosci Lett] 1996 May 17; Vol. 209 (3), pp. 193-6. - Publication Year :
- 1996
-
Abstract
- Oxidative stress is thought to play a key role both in the neurotoxin MPTP- and manganese (Mn)-induced neurotoxicity and in apoptotic cell death. In the present study, we report that Mn and the MPTP analogue 1-methyl-4-(2'-ethylphenyl)-1,2,3,6-tetrahydropyridine (2'Et-MPTP), which is metabolized by MAO-A to 1-methyl-4-(2'-ethylphenyl)-pyridinium ion (at concentrations of 0.5 and 1.0 mM), induced apoptosis in PC12 cells. Apoptosis was tested by terminal deoxynucleotidyl transferase-mediated 2'-deoxy-uridine-5'-triphosphate nick end labelling (TUNEL) technique, flow cytometry and fluorescence microscopy. Both Mn and 2'Et-MPTP induced also a time-dependent decrease in cell viability, as determined by the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Only Mn-induced apoptosis and decrease in cell viability were inhibited by the antioxidant ascorbic acid. We conclude that apoptosis may be an important mechanism of cell death in MPTP- and Mn-induced parkinsonism. However, an oxidative stress mechanism may be recognized only in the Mn-induced apoptosis.
- Subjects :
- Animals
Ascorbic Acid pharmacology
Cell Survival drug effects
Flow Cytometry
Genetic Techniques
Manganese antagonists & inhibitors
Microscopy, Fluorescence
Oxidative Stress drug effects
PC12 Cells
Rats
Uridine Triphosphate genetics
Uridine Triphosphate metabolism
Apoptosis drug effects
Manganese pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0304-3940
- Volume :
- 209
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Neuroscience letters
- Publication Type :
- Academic Journal
- Accession number :
- 8736643
- Full Text :
- https://doi.org/10.1016/0304-3940(96)12645-8