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Use of excretory/secretory antigens for the serodiagnosis of Anoplocephala perfoliata cestodosis.
- Source :
-
Veterinary parasitology [Vet Parasitol] 1996 Feb; Vol. 61 (3-4), pp. 239-47. - Publication Year :
- 1996
-
Abstract
- Whole worm extract (WWE) and excretory/secretory (E/S) antigens of Anoplocephala perfoliata were characterised by SDS-PAGE and their use in the serodiagnosis of equine cestodosis was evaluated. An enzyme-linked immunosorbent assay (ELISA) was used to compare WWE and E/S antigen as the capture layer in an antibody capture ELISA. E/S antigen gave the best differentiation between sera from tapeworm-positive and tapeworm-negative horses. The E/S-ELISA was optimised and validated against sera from horses of known tapeworm status. This assay gave a diagnostic sensitivity of 68% (n = 38) and a specificity in helminth-naive horses of 95% (n = 20). Western blot analysis of tapeworm-positive and tapeworm-negative sera revealed the immunoreactive proteins of E/S antigen to be those of 12 and 13 kDa.
- Subjects :
- Animals
Antibody Specificity
Cestoda immunology
Cestoda isolation & purification
Cestode Infections diagnosis
Electrophoresis, Polyacrylamide Gel
Enzyme-Linked Immunosorbent Assay methods
Enzyme-Linked Immunosorbent Assay veterinary
Horses
Immunoblotting
Reproducibility of Results
Sensitivity and Specificity
Serologic Tests methods
Species Specificity
Antibodies, Helminth blood
Antigens, Helminth blood
Cestode Infections veterinary
Horse Diseases
Subjects
Details
- Language :
- English
- ISSN :
- 0304-4017
- Volume :
- 61
- Issue :
- 3-4
- Database :
- MEDLINE
- Journal :
- Veterinary parasitology
- Publication Type :
- Academic Journal
- Accession number :
- 8720562
- Full Text :
- https://doi.org/10.1016/0304-4017(95)00837-3