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Evidence that filaggrin is a component of cornified cell envelopes in human plantar epidermis.

Authors :
Simon M
Haftek M
Sebbag M
Montézin M
Girbal-Neuhauser E
Schmitt D
Serre G
Source :
The Biochemical journal [Biochem J] 1996 Jul 01; Vol. 317 ( Pt 1), pp. 173-7.
Publication Year :
1996

Abstract

Cornified cell envelope (CE) is generated during the late stages of epidermal differentiation and is made up of proteins covalently linked together by transglutaminases. To determine whether filaggrin is a component of this structure in humans, we analysed highly purified CE from plantar stratum corneum. An immunoelectron microscopy analysis showed specific binding of four different anti-(pro)filaggrin monoclonal antibodies to the surface of the CE, proved previously to be free of non-covalently linked proteins. Moreover, the anti-filaggrin activity of one of the antibodies was absorbed by preincubation with the plantar CE, as determined by ELISA. Convincingly, fragments of CE produced by proteolytic digestion of the structures were stained by this antibody on immunoblots. These data provide direct evidence that filaggrin is a component of CE purified from human plantar stratum corneum. Cross-linking between CE and the filaggrin-containing fibrous matrix may enhance the structural cohesion of the corneocytes and thus the resistance of the stratum corneum.

Details

Language :
English
ISSN :
0264-6021
Volume :
317 ( Pt 1)
Database :
MEDLINE
Journal :
The Biochemical journal
Publication Type :
Academic Journal
Accession number :
8694761
Full Text :
https://doi.org/10.1042/bj3170173