Back to Search
Start Over
Complex of the soluble IL-11 receptor and IL-11 acts as IL-6-type cytokine in hepatic and nonhepatic cells.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1996 Jul 01; Vol. 157 (1), pp. 284-90. - Publication Year :
- 1996
-
Abstract
- The signaling functions of the membrane and soluble form of the mouse IL-11 receptor (mIL-11R) were compared in rat and human hepatoma cells, which have a low endogenous IL-11 response. The expression vectors encoding either the full length or a secretory form of the ligand binding subunit of mIL-11R together with IL-6-responsive reporter gene constructs were transiently transfected into the H-35 and HepG2 cells. An IL-11-specific stimulation of transcription was detected that was qualitatively similar to that mediated by the endogenous IL-6R. HepG2 cells were noted to synthesize constitutively IL-11, resulting in an autocrine stimulation of gene expression. Addition of COS cell-derived soluble mIL-11R to the hepatoma cell cultures prominently enhanced IL-11 regulation of transfected reporter gene constructs and expression of endogenous acute phase plasma protein genes. Similarly, the complex of soluble mIL-11R and IL-11 was capable of mediating an IL-6-type signaling in cells that are naturally deficient in IL-11 response as shown by the activation of STAT1 and STAT3 in mouse embryonal carcinoma cells and human T cells. The results indicate that the IL-11R can serve as a substitute to IL-6R in activating gene expression in target cells that are devoid of the appropriate ligand-binding receptor subunits.
- Subjects :
- Animals
Base Sequence
Carcinoma, Embryonal immunology
Humans
Interleukin-11 Receptor alpha Subunit
Interleukin-6 pharmacology
Macromolecular Substances
Mice
Molecular Sequence Data
Protein Binding immunology
Rats
Receptors, Interleukin-11
Solubility
T-Lymphocytes immunology
Transfection immunology
Tumor Cells, Cultured
Carcinoma, Hepatocellular immunology
Interleukin-11 physiology
Interleukin-6 analogs & derivatives
Liver Neoplasms immunology
Receptors, Interleukin physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 157
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 8683127