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Production of interferon gamma messenger RNA by cells of non-immune origin.
- Source :
-
Cytokine [Cytokine] 1995 Nov; Vol. 7 (8), pp. 793-8. - Publication Year :
- 1995
-
Abstract
- There is general agreement that IFN-gamma is produced only by cells of immune origin (T-cells, NK cells, and recently macrophages). However, indirect evidence has suggested that undetectable, low levels of IFN-gamma produced by cells of non-immune lineage, such as the murine line L-929, enhanced the antiviral activity of IFNs-alpha and/or beta following induction by agents such as the double stranded RNA poly ICLC. Since L-929 cells were one of the prototypic cell lines for studying murine IFN induction and action, we felt that it would be important to validate this observation by detection of the mRNA for IFN-gamma. If confirmed, it might indicate a role for IFN-gamma in non-immune cells. The present investigations revealed that mouse L-929 fibroblasts produce IFN-gamma message following exposure to conventional IFN-alpha/beta inducers such as poly ICLC or Newcastle disease virus. In addition, we found that IFN-gamma itself will induce its own message. We further show that this is not a phenomenon isolated to transformed cells since we found that normal mouse embryo fibroblasts also produced the message, however in a constitutive fashion.
- Subjects :
- Animals
Antibodies, Monoclonal pharmacology
Carboxymethylcellulose Sodium analogs & derivatives
Carboxymethylcellulose Sodium pharmacology
Embryo, Mammalian
Fibroblasts drug effects
Fibroblasts immunology
Interferon Inducers pharmacology
Interferon-alpha immunology
Interferon-beta immunology
L Cells
Mice
Organ Specificity
Poly I-C pharmacology
Polylysine analogs & derivatives
Polylysine pharmacology
Polymerase Chain Reaction
Recombinant Proteins
Interferon-gamma biosynthesis
Interferon-gamma pharmacology
RNA, Messenger biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 1043-4666
- Volume :
- 7
- Issue :
- 8
- Database :
- MEDLINE
- Journal :
- Cytokine
- Publication Type :
- Academic Journal
- Accession number :
- 8664446
- Full Text :
- https://doi.org/10.1006/cyto.1995.0095