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A cleavage-site-directed inhibitor of interleukin-1 beta-converting enzyme-like proteases inhibits apoptosis in primary cultures of rat hepatocytes.
- Source :
-
The Biochemical journal [Biochem J] 1996 Feb 15; Vol. 314 ( Pt 1), pp. 27-32. - Publication Year :
- 1996
-
Abstract
- Apoptosis induced in primary hepatocytes by transforming growth factor beta1 and staurosporine produced chromatin condensation, DNA cleavage is detected by in situ end-labelling, field inversion and conventional gel electrophoresis, and cell detachment. These effects were abolished by benzyloxycarbonyl-valinylalanylaspartylfluoromethyl ketone, a cleavage-site-directed inhibitor of interleukin-1beta-converting enzyme-like proteases, and this finding suggests that these enzymes are involved in liver apoptosis.
- Subjects :
- Alkaloids pharmacology
Animals
Caspase 1
Cell Nucleus drug effects
Cells, Cultured
Chromatin drug effects
Chromatin metabolism
DNA metabolism
Electrophoresis, Agar Gel
Liver drug effects
Male
Nucleosomes drug effects
Nucleosomes metabolism
Oligopeptides pharmacology
Protease Inhibitors pharmacology
Rats
Staurosporine
Transforming Growth Factor beta pharmacology
Amino Acid Chloromethyl Ketones pharmacology
Apoptosis drug effects
Cysteine Endopeptidases metabolism
Cysteine Proteinase Inhibitors pharmacology
Liver cytology
Subjects
Details
- Language :
- English
- ISSN :
- 0264-6021
- Volume :
- 314 ( Pt 1)
- Database :
- MEDLINE
- Journal :
- The Biochemical journal
- Publication Type :
- Academic Journal
- Accession number :
- 8660294
- Full Text :
- https://doi.org/10.1042/bj3140027