Back to Search
Start Over
Evaluation of ethanol on gastric epithelial restoration in vitro.
- Source :
-
Alcoholism, clinical and experimental research [Alcohol Clin Exp Res] 1996 Feb; Vol. 20 (1 Suppl), pp. 45A-46A. - Publication Year :
- 1996
-
Abstract
- Ethanol exerts damaging effects on gastric mucosa and delays ulcer healing. To investigate the effect of ethanol on the wound repairing process, we used a wound repair model using primary cultured gastric mucosal cells. A confluent monolayer gastric mucosal cell sheet consisting mainly of mucous cells was wounded to make a cell-free area of constant size. Cell-free area was restored with time after wounding and monitored every 12 hr using a computer image analyzer to observe epithelial cell restoration quantitatively in the presence and absence of ethanol (2.0%). It was found that, although the control wound was completely repaired in 36 to 48 hr, the group treated with 2.0% ethanol showed a significant delay of repair. In the control, 5-bromodeoxyuridine-positive cells appeared around the wound in 24 to 36 hr. In contrast, the group treated with 2.0% ethanol showed no 5-bromodeoxyuridine-positive cells during the experiment. In conclusion, 2.0% ethanol retarded the repair of gastric mucosal restoration by inhibiting the initial gastric cell migration, followed by inhibition of proliferation of cells.
Details
- Language :
- English
- ISSN :
- 0145-6008
- Volume :
- 20
- Issue :
- 1 Suppl
- Database :
- MEDLINE
- Journal :
- Alcoholism, clinical and experimental research
- Publication Type :
- Academic Journal
- Accession number :
- 8659688
- Full Text :
- https://doi.org/10.1111/j.1530-0277.1996.tb01727.x