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Nasal neutrophilia and release of myeloperoxidase induced by nasal challenge with platelet activating factor: different degrees of responsiveness in atopic and nonatopic subjects.
- Source :
-
The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 1996 Apr; Vol. 97 (4), pp. 947-54. - Publication Year :
- 1996
-
Abstract
- Background: Nasal challenge with platelet activating factor (PAF) is able to induce local neutrophilia, with a different degree of responsiveness in atopic subjects and in nonatopic subjects. We investigated whether nasal accumulation of neutrophils induced by PAF is accompanied by the release of neutrophil-derived mediators.<br />Methods: Nasal lavages were performed before and after challenge with PAF (500 nmol), lyso-PAF (500 nmol), and saline solution in 10 patients with allergic rhinitis and 10 normal subjects to evaluate changes in neutrophil counts and the release of myeloperoxidase (MPO) and immunoreactive leukotriene B4.<br />Results: PAF caused neutrophilia, which appeared after 30 minutes in atopic subjects and after 3 hours in nonatopic subjects. Furthermore, when compared with saline insufflation, PAF caused a significant release of MPO in the nasal lavage fluids collected 30 minutes, 3 hours, and 24 hours after challenge in atopic subjects and 3 hours after challenge in nonatopic subjects, with higher values in the former than in the latter. Neutrophil counts correlated with MPO levels in the nasal lavages collected after PAF challenge. A lower degree of neutrophilia was found 3 hours after stimulation with lyso-PAF in both groups of subjects, with a marginal release of MPO in atopic subjects only. No significant increase of immunoreactive leukotriene B4 levels in nasal lavages was found after challenge with either PAF or lyso-PAF.<br />Conclusion: These results indicate that PAF-induced neutrophilia in the nose is accompanied by the release of MPO, which appears earlier and is more marked in atopic subjects than in nonatopic subjects.
- Subjects :
- Administration, Intranasal
Adult
Female
Humans
Hypersensitivity, Immediate enzymology
Hypersensitivity, Immediate pathology
Leukocyte Count
Leukocytosis chemically induced
Leukocytosis enzymology
Leukotriene B4 metabolism
Male
Nasal Lavage Fluid chemistry
Nasal Lavage Fluid cytology
Neutrophils immunology
Peroxidase metabolism
Platelet Activating Factor pharmacology
Rhinitis, Allergic, Seasonal enzymology
Rhinitis, Allergic, Seasonal immunology
Rhinitis, Allergic, Seasonal pathology
Sodium Chloride pharmacology
Hypersensitivity, Immediate immunology
Leukocytosis immunology
Nasal Lavage Fluid immunology
Neutrophils drug effects
Neutrophils enzymology
Peroxidase drug effects
Platelet Activating Factor administration & dosage
Subjects
Details
- Language :
- English
- ISSN :
- 0091-6749
- Volume :
- 97
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Journal of allergy and clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 8655890
- Full Text :
- https://doi.org/10.1016/s0091-6749(96)80069-8