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A v-SNARE implicated in intra-Golgi transport.
- Source :
-
The Journal of cell biology [J Cell Biol] 1996 May; Vol. 133 (3), pp. 507-16. - Publication Year :
- 1996
-
Abstract
- We report the identification of a putative v-SNARE (GOS-28), localized primarily to transport vesicles at the terminal rims of Golgi stacks. In vitro, GOS-28, A Golgi SNARE of 28 kD, is efficiently packaged into Golgi-derived vesicles, which are most likely COPI coated. Antibodies directed against GOS-28 block its ability to bind alpha-SNAP, partially inhibit transport from the cis to the medial cisternae, and do not inhibit budding of COP-coated vesicles, but do accumulate docked uncoated vesicles.
- Subjects :
- Amino Acid Sequence
Animals
Antibody Specificity
Base Sequence
Binding, Competitive physiology
Biological Transport physiology
CHO Cells chemistry
CHO Cells metabolism
CHO Cells ultrastructure
Carrier Proteins immunology
Carrier Proteins metabolism
Cell Fractionation
Coated Vesicles chemistry
Cricetinae
Cytoplasm chemistry
Golgi Apparatus chemistry
Golgi Apparatus ultrastructure
Intracellular Membranes chemistry
Membrane Proteins immunology
Membrane Proteins isolation & purification
Microscopy, Immunoelectron
Molecular Sequence Data
Nerve Tissue Proteins isolation & purification
Nerve Tissue Proteins metabolism
SNARE Proteins
Soluble N-Ethylmaleimide-Sensitive Factor Attachment Proteins
Golgi Apparatus metabolism
Membrane Proteins metabolism
Vesicular Transport Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 133
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 8636227
- Full Text :
- https://doi.org/10.1083/jcb.133.3.507