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Expression and cellular localization of rab28 mRNA and Rab28 protein during maize embryogenesis.
- Source :
-
The Plant journal : for cell and molecular biology [Plant J] 1996 Apr; Vol. 9 (4), pp. 549-57. - Publication Year :
- 1996
-
Abstract
- The maize abscisic acid (ABA) responsive gene rab28, has been shown to be ABA-inducible in embryos and vegetative tissues. A polyclonal antiserum was raised against Rab28 protein. Using immunoblotting and immunoprecipitation, the antiserum specifically recognized a protein of about 30 kDa and pl 6 which is in close agreement with the molecular weight and pl predicted by the deduced amino acid sequence. The rab28 gene product accumulated during late embryogenesis. In vegetative tissues, dehydration stress induced rab28 gene expression both in the light and in the dark. The spatial and temporal pattern of rab28 mRNA expression during embryogenesis was investigated by in situ hybridization using digoxigenin-labelled rab28 probes, and the immunochemical localization of Rab28 protein using anti-Rab28 antibodies. Expression of rab28 mRNA is restricted to provascular tissues in young embryos, and at later stages of development the most prevalent accumulation occurred in meristem and in the vascular elements of the plumule, root and scutellum. Using immunoelectron microscopy the Rab28 protein has been located in the nucleolus of different cell types. In light of these results the stress regulation of rab28 and a likely role for this protein during late embryogenesis are discussed.
- Subjects :
- Antibody Specificity
Cell-Free System
Darkness
In Situ Hybridization
Light
Plant Proteins genetics
Plant Proteins immunology
Plant Proteins isolation & purification
Protein Biosynthesis
RNA, Messenger analysis
RNA, Plant analysis
Seeds anatomy & histology
Seeds genetics
Tissue Distribution
Transcription, Genetic
Zea mays anatomy & histology
Zea mays genetics
Gene Expression Regulation, Plant
Plant Proteins biosynthesis
Seeds metabolism
Zea mays metabolism
rab GTP-Binding Proteins
Subjects
Details
- Language :
- English
- ISSN :
- 0960-7412
- Volume :
- 9
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- The Plant journal : for cell and molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 8624517
- Full Text :
- https://doi.org/10.1046/j.1365-313x.1996.09040549.x