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Binding of HIV-1 virions or gp120-anti-gp120 immune complexes to HIV-1-infected quiescent peripheral blood mononuclear cells reveals latent infection.
- Source :
-
Journal of immunology (Baltimore, Md. : 1950) [J Immunol] 1996 May 15; Vol. 156 (10), pp. 3994-4004. - Publication Year :
- 1996
-
Abstract
- HIV-1-infected quiescent CD4+ cells harbor the virus in an inactive state until subsequent activation. The possibility that HIV-1 itself and the virus envelope glycoprotein 120 (gp120) might be important agents of this activation was investigated. The present data indicate that binding of heat-inactivated HIV-1 (iHIV-1) to infected resting PBMCs was sufficient to activate NF-kappa B and AP-1, to induce transition from the G0/G1 stage of the cell cycle to the S/G2/M stage, to induce cell surface expression of CD25, to stimulate provirus integration, and to commit cells to produce virus. The cumulative amount of HIV-1 produced by iHIV-1-stimulated cells strictly depended on the concentration of p24gag in the virion preparations used for stimulation. Moreover, virus production was not evidenced in infected resting cells exposed to iHIV-1 previously incubated with soluble CD4 (sCD4), indicating that activation requires a contact between HIV-1 envelope glycoproteins and cell surface CD4. Although soluble gp120 did not stimulate virus production, we found that transition to the S/G2/M stage of the cell cycle, cell surface expression of activation Ags, and virus production were stimulated by cross-linking of CD4 by gp120-anti-gp120 immune complexes. Finally, incubation of gp120-anti-gp120 immune complexes with sCD4 inhibited these effects. These findings suggest that virions and gp120 anti-gp120 immune complexes found in infected patients at all times of infection can stimulate virus production in CD4+ cells harboring HIV-1 in an inducible state.
- Subjects :
- Base Sequence
CD8-Positive T-Lymphocytes immunology
Genome, Viral
HIV Envelope Protein gp120 metabolism
HIV-1 immunology
Humans
Interphase immunology
Leukocytes, Mononuclear immunology
Lymphocyte Activation
Lymphocyte Depletion
Molecular Sequence Data
NF-kappa B metabolism
Transcription Factor AP-1 metabolism
Transcription, Genetic immunology
Virion immunology
Virus Integration immunology
Antigen-Antibody Complex metabolism
HIV Antibodies metabolism
HIV Envelope Protein gp120 immunology
HIV Infections immunology
HIV-1 metabolism
Leukocytes, Mononuclear metabolism
Virion metabolism
Virus Latency immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0022-1767
- Volume :
- 156
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- Journal of immunology (Baltimore, Md. : 1950)
- Publication Type :
- Academic Journal
- Accession number :
- 8621941