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Interleukin-6 stimulates neutrophil production of platelet-activating factor.
- Source :
-
Journal of leukocyte biology [J Leukoc Biol] 1996 Apr; Vol. 59 (4), pp. 569-74. - Publication Year :
- 1996
-
Abstract
- Interleukin-6 (IL-6) is an integral mediator of the acute phase response to injury and infection; an exaggerated IL-6 response has been associated with adverse clinical events. The precise role of IL-6 is unclear, but it appears capable of modulating the functional repertoire of mature neutrophils (PMNs). Our previous work demonstrated that IL-6 -stimulated PMNs are primed by lower concentrations of platelet-activating factor (PAF) than nonstimulated PMNs. Recently, we have found that IL-6 suppresses PMN apoptosis via a PAF-like mechanism. We hypothesized that IL-6 stimulates PMNs to produce PAF. PMNs isolated from healthy human donors were incubated with IL-6 (0.1-100 ng/ml) at 37 degrees C. Lipid production was measured by use of thin-layer chromatography, and PAF quantitated with a scintillation proximity assay. IL-6 (1 and 10 ng/ml) stimulated PMNs to produce increase quantities of PAF. PAF production was associated with an increase in PMN cytosolic calcium. These data may provide mechanistic insight into IL-6 regulation of PMN-mediated cytotoxicity and the role of PAF in mediating IL-6 effects on PMNs.
- Subjects :
- Amino Acid Sequence
Calcium blood
Cells, Cultured
Chromatography, Thin Layer
Cytosol metabolism
Humans
Lipids blood
Molecular Sequence Data
Signal Transduction drug effects
Stimulation, Chemical
Interleukin-6 pharmacology
Neutrophils drug effects
Neutrophils metabolism
Platelet Activating Factor biosynthesis
Subjects
Details
- Language :
- English
- ISSN :
- 0741-5400
- Volume :
- 59
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of leukocyte biology
- Publication Type :
- Academic Journal
- Accession number :
- 8613706
- Full Text :
- https://doi.org/10.1002/jlb.59.4.569