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Retinoylation of the type II cAMP-binding regulatory subunit of cAMP-dependent protein kinase is increased in psoriatic human fibroblasts.
- Source :
-
Journal of cellular physiology [J Cell Physiol] 1996 May; Vol. 167 (2), pp. 196-203. - Publication Year :
- 1996
-
Abstract
- Previously, we have reported a defect in the cAMP-dependent protein kinases (cAMP-PK) in psoriatic cells (i.e., a decrease in 8-azido-[32P]cAMP binding to the regulatory subunits and a decrease in phosphotransferase activity) which is rapidly reversed with retinoic acid (RA) treatment of these cells. This led us to examine a possible direct interaction between retinoids and the RI and RII regulatory subunits through retinoylation. Retinoylation of RI and RII present in normal and psoriatic human fibroblasts was analysed by [3H]RA treatment of these cells, followed either by chromatographic separation of the regulatory subunits or by their specific immunoprecipitation. These studies indicated that RI and RII can be retinoylated. [3H]RA labeling of the RII subunit was significantly (P < 0.005) greater in psoriatic fibroblasts (nine subjects; mean 7.47 relative units +/- 1.37 SEM) compared to normal fibroblasts (eight subjects; mean 2.46 relative +/- 0.49 SEM). [3H]RA labeling of and the increase in 8-azido-[32P]-binding to the RI and RII subunit in psoriatic fibroblasts showed a similar time course. This suggests that the rapid effect of retinoic acid treatment to enhance 8-azido-[32P]-cAMP binding to the RI and RII in psoriatic fibroblasts may be due, in part, to covalent modification of the regulatory subunits by retinoylation.
- Subjects :
- Affinity Labels
Autoradiography
Azides
Blotting, Western
Cell Fractionation
Chromatography
Cyclic AMP analogs & derivatives
Cyclic AMP-Dependent Protein Kinase Type II
Fibroblasts cytology
Fibroblasts drug effects
Humans
Phosphorus Radioisotopes
Protein Binding physiology
Skin cytology
Skin drug effects
Time Factors
Tretinoin metabolism
Tritium
Cyclic AMP-Dependent Protein Kinases metabolism
Fibroblasts enzymology
Psoriasis enzymology
Tretinoin pharmacology
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9541
- Volume :
- 167
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of cellular physiology
- Publication Type :
- Academic Journal
- Accession number :
- 8613459
- Full Text :
- https://doi.org/10.1002/(SICI)1097-4652(199605)167:2<196::AID-JCP2>3.0.CO;2-K