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Use of proZZ-obelin fusion protein in bioluminescent immunoassay.
- Source :
-
Biochemical and biophysical research communications [Biochem Biophys Res Commun] 1996 Feb 15; Vol. 219 (2), pp. 475-9. - Publication Year :
- 1996
-
Abstract
- Obelin is a photoprotein that emits light by Ca2+-binding. To develop a bioluminescent immunoassay based on the light emission property of obelin, we have expressed the apoobelin fusion protein with ZZ-domain of S. aureus protein A in E. coli by recombinant DNA techniques. The proZZ-obelin expressed was purified by one-step affinity chromatography on IgG-Agarose. The purified proZZ-obelin has both the luminescent activity of obelin and the IgG-binding ability of ZZ-domain. The specific activity of fusion protein was 8.5 X 10(15) photons per mg of protein.
- Subjects :
- Animals
Escherichia coli
Humans
Kinetics
Luminescent Measurements
Mice
Plasmids
Rabbits
Recombinant Fusion Proteins isolation & purification
Recombinant Fusion Proteins metabolism
Restriction Mapping
Antibodies, Bacterial analysis
Calcium metabolism
Immunoglobulin G analysis
Immunoglobulins analysis
Luminescent Proteins isolation & purification
Luminescent Proteins metabolism
Tuberculosis immunology
Subjects
Details
- Language :
- English
- ISSN :
- 0006-291X
- Volume :
- 219
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Biochemical and biophysical research communications
- Publication Type :
- Academic Journal
- Accession number :
- 8605012
- Full Text :
- https://doi.org/10.1006/bbrc.1996.0258