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The Bacillus subtilis response regulator Spo0A stimulates transcription of the spoIIG operon through modification of RNA polymerase promoter complexes.
- Source :
-
Journal of molecular biology [J Mol Biol] 1996 Mar 01; Vol. 256 (3), pp. 436-48. - Publication Year :
- 1996
-
Abstract
- Sporulation in Bacillus subtilis is dependent on the response regulator Spo0A, which both represses and activates transcription in vitro. The activity of Spo0A is increased by phosphorylation. We previously demonstrated that the phosphorylation increased the ability of Spo0A to stimulate in vivo transcription from the promoter for the spoIIG operon, one of the operons known to be regulated by Spo0A in vivo. In the work reported here we have examined the kinetics of transcription initiation at the spoIIG operon promoter using a single round transcription assay and the kinetics of formation of spoIIG promoter-RNA polymerase complexes using DNase I footprinting. Both the kinetic assays and the footprint assays indicated that the initial binding of the polymerase to the template was not dependent on the presence of Spo0A. The phosphorylated form of Spo0A stimulated the rate of initiation by affecting a step that occurred after the initial interaction of the polymerase with the template. Phosphorylation of Spo0A may stimulate transcription by modifying preinitiation complexes containing the polymerase and the promoter.
- Subjects :
- Bacillus subtilis physiology
Base Sequence
DNA Footprinting
DNA, Bacterial metabolism
DNA-Directed RNA Polymerases antagonists & inhibitors
Deoxyribonuclease I
Enzyme Inhibitors pharmacology
Gene Expression Regulation, Bacterial drug effects
Gene Expression Regulation, Bacterial physiology
Heparin pharmacology
Kinetics
Molecular Sequence Data
Nucleotides pharmacology
Operon genetics
Phosphorylation
Spores, Bacterial
Transcription, Genetic genetics
Bacillus subtilis genetics
Bacterial Proteins metabolism
DNA-Directed RNA Polymerases metabolism
Promoter Regions, Genetic genetics
Sigma Factor genetics
Transcription Factors genetics
Transcription Factors metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0022-2836
- Volume :
- 256
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Journal of molecular biology
- Publication Type :
- Academic Journal
- Accession number :
- 8604129
- Full Text :
- https://doi.org/10.1006/jmbi.1996.0099