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Protection of conjugated linoleic acids against 2-amino-3- methylimidazo[4,5-f]quinoline-induced colon carcinogenesis in the F344 rat: a study of inhibitory mechanisms.
- Source :
-
Carcinogenesis [Carcinogenesis] 1995 Dec; Vol. 16 (12), pp. 3037-43. - Publication Year :
- 1995
-
Abstract
- Grilled ground beef contains a number of heterocyclic amine carcinogens, such as 2-amino-3-methylimidazo[4,5-f] quinoline (IQ), as well as anticarcinogenic conjugated linoleic acids (CLA). In the present study, CLA was administered to male F344 rats by gavage on alternating days in weeks 1-4, while IQ was given by gavage every other day in weeks 3 and 4 (100 mg/kg body wt). Rats were killed 6 h after the final carcinogen dose 16 in order to score colonic aberrant crypt foci (ACF). In the ACF study, CLA had no effect on the size of the foci, but inhibited significantly (P < 0.05) the number of ACF/colon, from 4.3 +/- 2.4 in controls to 1.1 +/- 1.3 in CLA-treated rats (mean +/- SD, n = 10). Rats given CLA also had significantly lower IQ-DNA adducts in the colon as determined by 32P-postlabeling analysis; relative adduct labeling levels (RAL x 10(7) for the major adduct were 9.13 +/- 2.6 in controls versus 5.42 +/- 1.8 in CLA-treated animals (P < 0.05). Mechanism studies indicated that CLA and other fatty acids interact with certain heterocyclic amines in a manner consistent with substrate-ligand binding. However, no such interaction occurred with IQ, and CLA failed to inhibit significantly the mutagenicity of N-hydroxy-IQ in the Salmonella assay. Liver microsomes from CLA-treated rats exhibited lower activities for dealkylation of 7-ethoxyresorufin and methoxyresorufin and activated IQ to DNA binding species less effectively than microsomes from control animals. Direct addition of CLA to the in vitro incubation inhibited IQ-DNA binding and was associated with increased recovery of unmetabolized parent compound. In the Salmonella assay, CLA inhibited the mutagenic activity of IQ in the presence of S9 or ram seminal vesicle microsomes. Collectively, these results support a mechanism involving inhibition of carcinogen activation by CLA, as opposed to direct interaction with the procarcinogen, scavenging of electrophiles or selective induction of phase I detoxification pathways.
- Subjects :
- Animals
Antimutagenic Agents therapeutic use
Carcinogens antagonists & inhibitors
Colonic Neoplasms chemically induced
Colonic Neoplasms enzymology
DNA Adducts
Enzyme Induction
Linoleic Acid
Male
NADH, NADPH Oxidoreductases antagonists & inhibitors
NADH, NADPH Oxidoreductases biosynthesis
NADH, NADPH Oxidoreductases metabolism
NADPH-Ferrihemoprotein Reductase
Quinolines antagonists & inhibitors
Rats
Rats, Inbred F344
Substrate Specificity
Anticarcinogenic Agents therapeutic use
Carcinogens toxicity
Colonic Neoplasms prevention & control
Linoleic Acids therapeutic use
Quinolines toxicity
Subjects
Details
- Language :
- English
- ISSN :
- 0143-3334
- Volume :
- 16
- Issue :
- 12
- Database :
- MEDLINE
- Journal :
- Carcinogenesis
- Publication Type :
- Academic Journal
- Accession number :
- 8603482
- Full Text :
- https://doi.org/10.1093/carcin/16.12.3037