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Chromosome localization of human genes for clathrin adaptor polypeptides AP2 beta and AP50 and the clathrin-binding protein, VCP.
- Source :
-
Genomics [Genomics] 1995 Nov 01; Vol. 30 (1), pp. 94-7. - Publication Year :
- 1995
-
Abstract
- Clathrin-coated vesicles, involved in endocytosis and Golgi processing, have a surface lattice containing clathrin triskelia and stoichiometric amounts of additional components termed "assembly proteins," or APs. The AP form at the plasma membrane, AP2, is composed of two large subunits of 100-115 kDa, denoted AP2 alpha and AP2 beta, a medium chain of 50 kDa, designated AP50, and a small chain. We have determined human chromosomal locations of genes for a large AP2 beta (CLAPB1) and a medium (CLAPM1) AP subunit and of a novel clathrin-binding protein, VCP, that binds clathrin simultaneously with APs. Chromosomal in situ hybridization of a human genomic clone demonstrated that the CLAPM1 gene mapped to chromosome region 3q28. The gene for the CLAPB1 large subunit was mapped to 17q11.2-q12 by PCR amplification of an AP2 beta fragment from a panel of rodent-human hybrid DNAs. To map the human VCP sequence, a human-specific probe was made by RT-PCR of human mRNA using oligonucleotide primers from conserved regions of the porcine sequence. The amplified human fragment served as probe on Southern blots of hybrid DNAs to determine that the human VCP locus maps to chromosome region 9pter-q34.
- Subjects :
- Adaptor Protein Complex 2
Adaptor Protein Complex alpha Subunits
Adaptor Proteins, Vesicular Transport
Animals
Base Sequence
Blotting, Southern
Chromosome Mapping
Chromosomes, Human, Pair 17
Chromosomes, Human, Pair 3
Chromosomes, Human, Pair 9
DNA Primers
HSC70 Heat-Shock Proteins
Humans
Hybrid Cells
In Situ Hybridization
Mice
Molecular Sequence Data
Rats
Adaptor Protein Complex mu Subunits
Carrier Proteins genetics
HSP70 Heat-Shock Proteins
Membrane Proteins genetics
Nerve Tissue Proteins genetics
Phosphoproteins genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0888-7543
- Volume :
- 30
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Genomics
- Publication Type :
- Academic Journal
- Accession number :
- 8595912
- Full Text :
- https://doi.org/10.1006/geno.1995.0016