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Reduced recovery of a Cryptococcus neoformans adherence mutant from a rat model of cryptococcosis.
Reduced recovery of a Cryptococcus neoformans adherence mutant from a rat model of cryptococcosis.
- Source :
-
Canadian journal of microbiology [Can J Microbiol] 1995 Apr-May; Vol. 41 (4-5), pp. 428-32. - Publication Year :
- 1995
-
Abstract
- Stable mutants of Cryptococcus neoformans (strain CSF-1) induced by treatment with ultraviolet light and nitrosoguanidine were isolated that demonstrated reduced adherence to glial cells in culture. Adherence of the mutants, as measured by a radiometric assay, was reduced by 50-70% of that attained for the parent CSF-1 strain. The adherence mutants appeared to be phenotypically similar to the CSF-1 strain. However, all but one mutant (designated as CSF-23) demonstrated slightly slower growth rates than the wild-type strain. The CSF-1 and CSF-23 strains were injected intravenously and intratracheally into normal rats and rats immunosuppressed by cyclophosphamide treatment, and the organ distribution and recovery of viable yeasts determined over 2-96 h. During this relatively short period of observation the majority of the yeasts were localized in the lungs. By either route of injection, the recovery of the CSF-23 adherence mutant was reduced by as much as 90% of that obtained for the wild-type strain. The results indicated that host cell adherence may be important for the persistence of cryptococci in tissue and that further studies with the adherence mutants are warranted.
- Subjects :
- Animals
Cell Line
Cryptococcosis genetics
Cryptococcus neoformans growth & development
Cryptococcus neoformans radiation effects
Immunosuppression Therapy
Lung microbiology
Male
Methylnitronitrosoguanidine adverse effects
Mutagenesis
Neuroglia metabolism
Rats
Rats, Sprague-Dawley
Time Factors
Tissue Distribution
Ultraviolet Rays
Cell Adhesion
Cryptococcosis microbiology
Cryptococcus neoformans genetics
Mutation
Subjects
Details
- Language :
- English
- ISSN :
- 0008-4166
- Volume :
- 41
- Issue :
- 4-5
- Database :
- MEDLINE
- Journal :
- Canadian journal of microbiology
- Publication Type :
- Academic Journal
- Accession number :
- 8590419
- Full Text :
- https://doi.org/10.1139/m95-057