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Reduced recovery of a Cryptococcus neoformans adherence mutant from a rat model of cryptococcosis.

Reduced recovery of a Cryptococcus neoformans adherence mutant from a rat model of cryptococcosis.

Authors :
Merkel GJ
Scofield BA
Rescorla FJ
Yang R
Grosfeld JL
Source :
Canadian journal of microbiology [Can J Microbiol] 1995 Apr-May; Vol. 41 (4-5), pp. 428-32.
Publication Year :
1995

Abstract

Stable mutants of Cryptococcus neoformans (strain CSF-1) induced by treatment with ultraviolet light and nitrosoguanidine were isolated that demonstrated reduced adherence to glial cells in culture. Adherence of the mutants, as measured by a radiometric assay, was reduced by 50-70% of that attained for the parent CSF-1 strain. The adherence mutants appeared to be phenotypically similar to the CSF-1 strain. However, all but one mutant (designated as CSF-23) demonstrated slightly slower growth rates than the wild-type strain. The CSF-1 and CSF-23 strains were injected intravenously and intratracheally into normal rats and rats immunosuppressed by cyclophosphamide treatment, and the organ distribution and recovery of viable yeasts determined over 2-96 h. During this relatively short period of observation the majority of the yeasts were localized in the lungs. By either route of injection, the recovery of the CSF-23 adherence mutant was reduced by as much as 90% of that obtained for the wild-type strain. The results indicated that host cell adherence may be important for the persistence of cryptococci in tissue and that further studies with the adherence mutants are warranted.

Details

Language :
English
ISSN :
0008-4166
Volume :
41
Issue :
4-5
Database :
MEDLINE
Journal :
Canadian journal of microbiology
Publication Type :
Academic Journal
Accession number :
8590419
Full Text :
https://doi.org/10.1139/m95-057