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Determination of beta-(1-3),(1-4)-D-glucans in barley by reversed-phase high-performance liquid chromatography.
- Source :
-
Journal of chromatography. A [J Chromatogr A] 1995 Dec 22; Vol. 718 (2), pp. 291-7. - Publication Year :
- 1995
-
Abstract
- An HPLC method for the determination of beta-glucan in barley was developed. The beta-glucan was hydrolysed with lichenase [endo-beta-(1-3),(1-4)-D-glucan-4-glucanhydrolase from Bacillus subtilis] to oligosaccharides, which were analysed by reversed-phase HPLC using water as the mobile phase at a flow-rate of 0.7 ml/min. The separation of the oligosaccharides was performed in a C18 stainless-steel column (Spherisorb ODS-2) with 5-microns particles in less than 10 min, with a refractive index detection.
- Subjects :
- Carbohydrate Conformation
Carbohydrate Sequence
Glycoside Hydrolases metabolism
Hydrolysis
Molecular Sequence Data
Oligosaccharides analysis
Oligosaccharides metabolism
Refractometry
Reproducibility of Results
Chromatography, High Pressure Liquid
Glucans analysis
Hordeum chemistry
beta-Glucans
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9673
- Volume :
- 718
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Journal of chromatography. A
- Publication Type :
- Academic Journal
- Accession number :
- 8589819
- Full Text :
- https://doi.org/10.1016/0021-9673(95)00694-x