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Mice immunized with a dengue type 2 virus E and NS1 fusion protein made in Escherichia coli are protected against lethal dengue virus infection.
- Source :
-
Vaccine [Vaccine] 1995 Sep; Vol. 13 (13), pp. 1251-8. - Publication Year :
- 1995
-
Abstract
- A gene fragment encoding the C-terminal 204 amino acids (AA) from the structural envelope glycoprotein (E) and the N-terminal 65 AA from non-structural protein one (NS1) of dengue type 2 virus (DEN-2) was expressed in Escherichia coli (E. coli) as a fusion protein with staphylococcal protein A. The recombinant fusion protein was purified and analysed for its antigenicity, its immunogenicity and its ability to protect mice against lethal challenge with live DEN-2 virus. The recombinant protein was found to be reactive with anti-DEN-2 polyclonal and monoclonal antibodies. Mice immunized with the purified fusion protein made anti-DEN-2 antibodies measured by the hemagglutination-inhibition (HI) and neutralization (N) tests, and were protected against lethal challenge with DEN-2 virus administered by intracranial inoculation.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Clone Cells
Dengue Virus genetics
Escherichia coli
Genome, Viral
Mice
Mice, Inbred BALB C
Mice, Inbred ICR
Molecular Sequence Data
Recombinant Fusion Proteins biosynthesis
Viral Envelope Proteins biosynthesis
Viral Nonstructural Proteins biosynthesis
Dengue prevention & control
Dengue Virus immunology
Immunization
Viral Envelope Proteins genetics
Viral Nonstructural Proteins genetics
Viral Vaccines
Subjects
Details
- Language :
- English
- ISSN :
- 0264-410X
- Volume :
- 13
- Issue :
- 13
- Database :
- MEDLINE
- Journal :
- Vaccine
- Publication Type :
- Academic Journal
- Accession number :
- 8578812
- Full Text :
- https://doi.org/10.1016/0264-410x(94)00059-v