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Monoclonal antibody blocking tests for the detection of HSV-1- and HSV-2-specific humoral responses: comparison with western blot assay.
- Source :
-
Journal of virological methods [J Virol Methods] 1995 Sep; Vol. 55 (1), pp. 27-35. - Publication Year :
- 1995
-
Abstract
- Monoclonal antibody blocking radioimmunoassays (MAb block RIAs) which detect specific humoral responses to each of the two human herpes simplex virus (HSV) types are described. RIAs were compared with Western blot assay (WBA) in a blind study of 64 sera obtained from clinically well-documented cases of genital herpes. WBA and MAb block RIA each detected HSV-1 antibodies in 16/17 (94%) sera from confirmed HSV-1 genital infections (first episodes and recurrent infections). Detection of HSV-2 antibody in 21 sera from HSV-2 first episodes was more effective by WBA which identified homologous antibody in 19 (96%), whereas MAb block RIA detected HSV-2 antibody in 16 (76%). HSV-2 antibody was detected in 24/25 (96%) sera from recurrent HSV-2 infections by WBA and by MAb block RIA, the highest degree of concordance for both methods. In addition, the MAb block RIA may be more effective in detecting the presence of HSV-1 antibody in sera from recurrent HSV-2 cases. Prevalence of HSV-1 and HSV-2 antibody was measured by the MAb block RIAs in 3 UK human study populations which consisted of 100 children/young adolescents, 104 adult blood donors and 80 genito-urinary medicine clinic attenders.
- Subjects :
- Adolescent
Adult
Animals
Antibodies, Monoclonal immunology
Antibodies, Viral blood
Blotting, Western
Child
Child, Preschool
Chlorocebus aethiops
Herpes Genitalis blood
Herpes Genitalis virology
Herpes Simplex blood
Herpes Simplex immunology
Herpesvirus 1, Human isolation & purification
Herpesvirus 2, Human isolation & purification
Humans
Single-Blind Method
Vero Cells
Antibodies, Viral immunology
Herpes Genitalis immunology
Herpes Simplex virology
Herpesvirus 1, Human immunology
Herpesvirus 2, Human immunology
Radioimmunoassay methods
Subjects
Details
- Language :
- English
- ISSN :
- 0166-0934
- Volume :
- 55
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Journal of virological methods
- Publication Type :
- Academic Journal
- Accession number :
- 8576306
- Full Text :
- https://doi.org/10.1016/0166-0934(95)00042-s