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Role of nitric oxide in induction of inflammatory fluid secretion by the mucosa of the feline gallbladder.
- Source :
-
Gastroenterology [Gastroenterology] 1996 Feb; Vol. 110 (2), pp. 598-606. - Publication Year :
- 1996
-
Abstract
- Background & Aims: Nitric oxide is synthesized from L-arginine and is metabolized to nitrate and nitrite. This study evaluates the effects of a pharmacological blockade of NO synthesis on fluid transport by the inflamed gallbladder mucosa.<br />Methods: Experiments were performed in cats with cholecystitis and in control animals. NO synthase activity was measured in gallbladder tissue; the enzyme was characterized by immunoblotting techniques and localized by immunofluorescence. Fluid transport and release of nitrate and nitrite by the gallbladder mucosa and bile and bile salt secretion from the liver were registered simultaneously in vivo.<br />Results: Fluid secretion in inflamed gallbladders was reversed to a net absorption in response to the NO synthase blockers N omega-nitro-L-arginine and aminoguanidine, and formation of nitrate was reduced. The effects were reversed by L-arginine. Increased levels of inducible NO synthase in inflamed gallbladders were shown by immunoblotting, by immunofluorescence (mainly in macrophages), and by Ca(2+)-independent [3H]citrulline formation from [3H]arginine. The NO synthase blockers had no effect on gallbladder fluid transport in normal gallbladders.<br />Conclusions: Increased levels of inducible NO synthase activity are shown in inflamed gallbladders, and a pharmacological blockade of this enzyme blocks fluid secretion and decreases nitrate release from the mucosa.
- Subjects :
- Analysis of Variance
Animals
Arginine analogs & derivatives
Arginine pharmacology
Bile metabolism
Bile Acids and Salts metabolism
Cats
Cholecystitis enzymology
Cholecystitis metabolism
Enzyme Inhibitors pharmacology
Gallbladder enzymology
Guanidines pharmacology
Immunohistochemistry
Mucous Membrane enzymology
Mucous Membrane metabolism
Nitrates metabolism
Nitric Oxide Synthase antagonists & inhibitors
Nitric Oxide Synthase metabolism
Nitroarginine
Body Fluids metabolism
Cholecystitis physiopathology
Gallbladder metabolism
Nitric Oxide physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0016-5085
- Volume :
- 110
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Gastroenterology
- Publication Type :
- Academic Journal
- Accession number :
- 8566609
- Full Text :
- https://doi.org/10.1053/gast.1996.v110.pm8566609