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Dominant role of mitochondria in clearance of large Ca2+ loads from rat adrenal chromaffin cells.
- Source :
-
Neuron [Neuron] 1996 Jan; Vol. 16 (1), pp. 219-28. - Publication Year :
- 1996
-
Abstract
- Cytosolic Ca2+ (Ca2+c) clearance from adrenal chromaffin cells was studied by whole-cell patch clamp and indo-1 Ca2+ photometry after influx of Ca2+ through voltage-dependent Ca2+ channels. We isolated the rates of Ca2+c clearance by several mechanisms using combinations of the following agents (with their expected targets): Li+ or TEA substituted for Na+ (Na(+)-Ca2+ exchange), 1 mM La3+ applied after the depolarization (Na(+)-Ca2+ exchange and plasma membrane Ca(2+)-ATPase), 1 microM thapsigargin (pumping into reticular stores), and 2 microM carbonyl cyanide m-chlorophenylhydrazone (uptake into mitochondria). Remarkably, whenever [Ca2+]c rose above approximately 500 nM, Ca2+c clearance by mitochondria exceeded clearance by either Na(+)-Ca2+ exchange or the Ca2+ pumps of the plasma and reticular membranes. As [Ca2+]c fell again, Ca2+ reemerged from mitochondria, prolonging the final return to basal levels.
- Subjects :
- Adrenal Medulla cytology
Adrenal Medulla embryology
Animals
Biological Transport drug effects
Biological Transport, Active drug effects
Calcium Channel Blockers pharmacology
Calcium-Transporting ATPases antagonists & inhibitors
Carbonyl Cyanide m-Chlorophenyl Hydrazone pharmacology
Carrier Proteins antagonists & inhibitors
Cell Lineage
Cells, Cultured
Intracellular Fluid metabolism
Intracellular Membranes drug effects
Intracellular Membranes metabolism
Lanthanum pharmacology
Lithium pharmacology
Male
Membrane Potentials drug effects
Neural Crest
Neurons metabolism
Patch-Clamp Techniques
Rats
Rats, Sprague-Dawley
Sodium-Calcium Exchanger
Terpenes pharmacology
Tetraethylammonium
Tetraethylammonium Compounds pharmacology
Thapsigargin
Adrenal Medulla metabolism
Calcium metabolism
Calcium-Transporting ATPases metabolism
Carrier Proteins metabolism
Mitochondria physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0896-6273
- Volume :
- 16
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Neuron
- Publication Type :
- Academic Journal
- Accession number :
- 8562086
- Full Text :
- https://doi.org/10.1016/s0896-6273(00)80038-0