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Nup120p: a yeast nucleoporin required for NPC distribution and mRNA transport.
- Source :
-
The Journal of cell biology [J Cell Biol] 1995 Dec; Vol. 131 (6 Pt 2), pp. 1659-75. - Publication Year :
- 1995
-
Abstract
- To extend our understanding of the mechanism by which the nuclear pore complex (NPC) mediates macromolecular transport across the nuclear envelope we have focused on defining the composition and molecular organization of the yeast NPC. Peptide sequence analysis of a polypeptide with a M(r) of approximately 100,000 present in a highly enriched yeast NPC fraction identified a novel yeast nucleoporin we term Nup120p. Nup120p corresponds to the open reading frame (ORF) YKL057c identified by the yeast genome sequencing project. The ORF predicts a protein with a calculated molecular mass of 120.5 kD containing two leucine zipper motifs, a short coiled-coil region and limited primary sequence similarity to Nup133p. Nup120p was localized to the NPC using a protein A-tagged chimera in situ by indirect immunofluorescence microscopy. Deletion of the NUP120 gene caused clustering of NPCs at one side of the nuclear envelope, moderate nucleolar fragmentation and slower cell growth. Transfer of nup120 delta cells to 37 degrees C resulted in the nuclear accumulation of poly(A)+ mRNA, extensive fragmentation of the nucleolus, spindle defects, and cell death.
- Subjects :
- Amino Acid Sequence
Base Sequence
Biological Transport genetics
Biomarkers
Fluorescent Antibody Technique
Gene Deletion
Genes, Fungal physiology
Microscopy, Electron
Molecular Sequence Data
Nuclear Envelope metabolism
Nuclear Envelope ultrastructure
Nuclear Proteins analysis
Nuclear Proteins genetics
RNA, Messenger metabolism
Sensitivity and Specificity
Temperature
Yeasts cytology
Yeasts ultrastructure
Nuclear Envelope chemistry
Nuclear Pore Complex Proteins
Nuclear Proteins isolation & purification
Saccharomyces cerevisiae Proteins
Yeasts genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 131
- Issue :
- 6 Pt 2
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 8557736
- Full Text :
- https://doi.org/10.1083/jcb.131.6.1659