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Isolation and characterization of cDNA clones for Humly9: the human homologue of mouse Ly9.
- Source :
-
Immunogenetics [Immunogenetics] 1996; Vol. 43 (1-2), pp. 13-9. - Publication Year :
- 1996
-
Abstract
- Ly9 is a mouse cell membrane antigen found on all lymphocytes and coded for by a gene that maps to chromosome 1. We previously described the isolation and characterization of a full-length cDNA clone for mouse Ly9. Using cross-species hybridization we isolated cDNA clones encoding the human homologue Humly9. Analysis of the predicted protein sequence suggests that the extra-cellular portion of the Humly9 molecules is composed of four Ig-like domains: a V domain (V) without disulphide bonds and a truncated C2 domain (tC2) with two disulphide bonds, a second V domain without disulphide bonds and a second tC2 with two disulphide bonds, i.e., as V-tC2-V-tC2. The gene encoding Humly9 was mapped to chromosome 1 by analysis of human/hamster hybrids, and more specifically to the 1q22 region by in situ hybridization. The protein sequence data support the view that Humly9 belongs to the immunoglobulin-superfamily subgroup which includes CD48, CD2, and LFA-3.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Chromosome Mapping
Chromosomes, Human, Pair 1
Cricetinae
DNA, Complementary genetics
Gene Library
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Mice
Molecular Sequence Data
Multigene Family
Sequence Alignment
Sequence Homology, Amino Acid
Species Specificity
Antigens, Ly genetics
Genes
Subjects
Details
- Language :
- English
- ISSN :
- 0093-7711
- Volume :
- 43
- Issue :
- 1-2
- Database :
- MEDLINE
- Journal :
- Immunogenetics
- Publication Type :
- Academic Journal
- Accession number :
- 8537117
- Full Text :
- https://doi.org/10.1007/BF00186599