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[The western immunoblotting technique in atypical situations of Rickettsia conorii infection. Presentation of 2 cases].
- Source :
-
Enfermedades infecciosas y microbiologia clinica [Enferm Infecc Microbiol Clin] 1993 Mar; Vol. 11 (3), pp. 139-42. - Publication Year :
- 1993
-
Abstract
- Background: During the last few years the application of Western immunoblotting in the study of human infection by Rickettsia conorii has led to the development of a new method of serologic diagnosis of the Mediterranean exanthematous fever.<br />Methods: The pattern of reactivity of serum samples sequentially obtained in the course of infection versus R. conorii antigens (Malish 7 strain) purified in discontinued density gradient and separated by SDS-PAGE was analyzed by Western immunoblotting.<br />Results: The presence of typical profiles of rickettsial infection similar to those observed in the common forms of presentation of the Mediterranean exanthematous fever was demonstrated in a case of accidental infection transmitted via aerosol and followed by an incomplete clinical picture after the early administration of specific antibiotic therapy and in a patient with signs and symptoms characteristic of Mediterranean exanthematous fever in whom the results of indirect immunofluorescence tests were repeatedly negative.<br />Conclusions: Western immunoblotting may be a specially useful technique as a complementary procedure in the laboratory to guide in the diagnosis of Mediterranean exanthematous fever in special situations.
Details
- Language :
- Spanish; Castilian
- ISSN :
- 0213-005X
- Volume :
- 11
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Enfermedades infecciosas y microbiologia clinica
- Publication Type :
- Academic Journal
- Accession number :
- 8499513