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Protein kinase C-activating domains of parathyroid hormone-related protein.
- Source :
-
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research [J Bone Miner Res] 1993 Apr; Vol. 8 (4), pp. 497-503. - Publication Year :
- 1993
-
Abstract
- N-terminal fragments of PTH-related protein (PTHrP), PTHrP-(1-34), and PTHrP-(1-40) stimulated both adenylyl cyclase and a mechanism that increases membrane-associated protein kinase C (PKC) activity in ROS 17/2 rat osteosarcoma cells. There were two peaks in the PKC response to the N-terminal PTHrP fragments: one peak was obtained with picomolar and the other with nanomolar PTHrP concentrations. The PKC-stimulating picomolar concentrations of the PTHrP fragments did not detectably stimulate adenylyl cyclase, but the nanomolar concentrations did. Since a similar two-peak response of PKC activity was obtained with PTHrP-(28-34), the single, N-terminal PKC activation domain of the PTHrP is in the same 28-34 region of the molecule as that of PTH despite this region having different primary amino acid sequences in the two hormones. Unlike PTH, PTHrP has a second PKC activation domain, as indicated by the ability of picomolar concentrations of the PTHrP-(107-111) fragment to stimulate maximally membrane-associated PKC activity in the osteosarcoma cells.
- Subjects :
- Adenylyl Cyclases metabolism
Amino Acid Sequence
Animals
Binding Sites
Bone and Bones enzymology
Enzyme Activation physiology
Humans
Molecular Sequence Data
Osteosarcoma
Parathyroid Hormone-Related Protein
Peptide Fragments
Phosphorylation
Rats
Tumor Cells, Cultured
Parathyroid Hormone physiology
Protein Kinase C metabolism
Proteins physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0884-0431
- Volume :
- 8
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
- Publication Type :
- Academic Journal
- Accession number :
- 8475799
- Full Text :
- https://doi.org/10.1002/jbmr.5650080414