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Elimination of L-A double-stranded RNA virus of Saccharomyces cerevisiae by expression of gag and gag-pol from an L-A cDNA clone.
- Source :
-
Journal of virology [J Virol] 1993 May; Vol. 67 (5), pp. 2764-71. - Publication Year :
- 1993
-
Abstract
- We report that expression of a nearly full-length cDNA clone of the L-A double-stranded RNA virus causes virus loss in a wild-type strain of Saccharomyces cerevisiae. We show that in this system exclusion of the L-A virus is independent of the presence of the packaging site or of cis sites for replication and transcription and completely dependent on expression of functional recombinant gag and gag-pol fusion protein. Thus, this exclusion is not explained in terms of overexpression of packaging signals. Mutation of the chromosomal SKI2 gene, known to repress the copy number of double-stranded RNA cytoplasmic replicons of S. cerevisiae, nearly eliminates the exclusion. We suggest that exclusion is due to competition by proteins expressed from the plasmid for a possibly limiting cellular factor. Our hypotheses on exclusion of L-A proteins may also apply to resistance to plant viruses produced by expression of viral replicases in transgenic plants.
- Subjects :
- Acetyltransferases
Amino Acid Sequence
Base Sequence
Fusion Proteins, gag-pol biosynthesis
Gene Products, gag biosynthesis
Genes, Viral genetics
Genetic Vectors genetics
Molecular Sequence Data
N-Terminal Acetyltransferase B
RNA Viruses growth & development
RNA, Double-Stranded genetics
RNA, Viral genetics
Viral Proteins genetics
Virus Replication
Fusion Proteins, gag-pol genetics
Gene Products, gag genetics
RNA Viruses genetics
Saccharomyces cerevisiae
Saccharomyces cerevisiae Proteins
Viral Interference genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0022-538X
- Volume :
- 67
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Journal of virology
- Publication Type :
- Academic Journal
- Accession number :
- 8474174
- Full Text :
- https://doi.org/10.1128/JVI.67.5.2764-2771.1993