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Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase.
- Source :
-
Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 1993 Feb 15; Vol. 90 (4), pp. 1498-502. - Publication Year :
- 1993
-
Abstract
- The association of host proteins with viral RNA replication proteins has been reported for a number of (+)-strand RNA viruses. However, little is known about the identity or function of these host proteins in viral replication. In this paper we report the characterization of a host protein associated with the RNA-dependent RNA polymerase (RdRp) from brome mosaic virus (BMV)-infected barley. A host protein was specifically and proportionally enriched with BMV RdRp activity through several purification steps. This RdRp-associated host protein reacted with an antiserum prepared against wheat germ eukaryotic translation initiation factor 3 (eIF-3). The RdRp-associated host protein, the p41 subunit of wheat germ eIF-3, and an antigenically related protein from rabbit reticulocyte lysates were all found to bind with high affinity and specificity to BMV-encoded protein 2a, which is involved in viral RNA replication. Moreover, addition of wheat germ eIF-3 or the p41 subunit from wheat germ to BMV RdRp gave a specific and reproducible 3-fold stimulation of (-)-strand RNA synthesis in vivo. These results suggest that the barley analog of eIF-3 subunit p41, or a closely related protein, associates with BMV RdRp in vivo and is involved in BMV RNA replication. This observation and the established role of translation factors in bacteriophage Q beta RdRp suggest that association with translation factors may be a general feature of RNA replication by (+)-strand RNA viruses.
- Subjects :
- Animals
Chromatography, Gel
Chromatography, Ion Exchange
Eukaryotic Initiation Factor-3
Hordeum microbiology
Kinetics
Molecular Weight
Mosaic Viruses isolation & purification
Peptide Initiation Factors isolation & purification
Peptide Initiation Factors metabolism
Plant Proteins isolation & purification
Protein Biosynthesis
RNA-Dependent RNA Polymerase isolation & purification
Rabbits
Reticulocytes metabolism
Triticum metabolism
Mosaic Viruses enzymology
Plant Proteins metabolism
RNA-Dependent RNA Polymerase metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0027-8424
- Volume :
- 90
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Proceedings of the National Academy of Sciences of the United States of America
- Publication Type :
- Academic Journal
- Accession number :
- 8434011
- Full Text :
- https://doi.org/10.1073/pnas.90.4.1498