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Hepatic and lipoprotein lipases selectively assayed in postheparin plasma.
- Source :
-
Clinical chemistry [Clin Chem] 1993 Feb; Vol. 39 (2), pp. 218-23. - Publication Year :
- 1993
-
Abstract
- Sensitive, reliable procedures are reported for the selective assay of lipoprotein lipase (LPL) and hepatic lipase (HL) in postheparin plasma samples. LPL is inhibited in the HL assay by inclusion of 0.76 mol/L sodium chloride in the substrate. In the LPL assay, specificity is attained by pretreating the sample with sodium dodecyl sulfate, which selectively denatures HL. This LPL method was validated by direct comparison with a procedure in which HL is inactivated by an antiserum to human HL. We used the described assays to quantify LPL and HL activity in 32 normal adults, demonstrating a clear sex difference for both enzymes. On average, the men displayed higher HL and lower LPL activities than did the women.
- Subjects :
- Adult
Apolipoprotein C-II
Apolipoproteins C pharmacology
Carbon Radioisotopes
Emulsions
Female
Gum Arabic
Humans
Lipase antagonists & inhibitors
Lipoprotein Lipase antagonists & inhibitors
Male
Phosphatidylcholines
Protein Denaturation
Reference Values
Scintillation Counting
Serum Albumin pharmacology
Sodium Chloride pharmacology
Sodium Dodecyl Sulfate pharmacology
Triolein
Heparin
Lipase blood
Lipoprotein Lipase blood
Liver enzymology
Subjects
Details
- Language :
- English
- ISSN :
- 0009-9147
- Volume :
- 39
- Issue :
- 2
- Database :
- MEDLINE
- Journal :
- Clinical chemistry
- Publication Type :
- Academic Journal
- Accession number :
- 8432009