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Cross-coupling of the NF-kappa B p65 and Fos/Jun transcription factors produces potentiated biological function.
- Source :
-
The EMBO journal [EMBO J] 1993 Oct; Vol. 12 (10), pp. 3879-91. - Publication Year :
- 1993
-
Abstract
- NF-kappa B and AP-1 represent distinct mammalian transcription factors that target unique DNA enhancer elements. The heterodimeric NF-kappa B complex is typically composed of two DNA binding subunits, NF-kappa B p50 and NF-kappa B p65, which share structural homology with the c-rel proto-oncogene product. Similarly, the AP-1 transcription factor complex is comprised of dimers of the c-fos and c-jun proto-oncogene products or of closely related proteins. We now demonstrate that the bZIP regions of c-Fos and c-Jun are capable of physically interacting with NF-kappa B p65 through the Rel homology domain. This complex of NF-kappa B p65 and Jun or Fos exhibits enhanced DNA binding and biological function via both the kappa B and AP-1 response elements including synergistic activation of the 5' long terminal repeat of the human immunodeficiency virus type 1. These findings support a combinatorial mechanism of gene regulation involving the unexpected cross-coupling of two different classes of transcription factors to form novel protein complexes exhibiting potentiated biological activity.
- Subjects :
- 3T3 Cells
Amino Acid Sequence
Animals
Blotting, Western
Cytoplasm metabolism
Electrophoresis, Polyacrylamide Gel
HeLa Cells
Humans
Macromolecular Substances
Mice
Molecular Sequence Data
Promoter Regions, Genetic
Proto-Oncogene Mas
Proto-Oncogene Proteins chemistry
Proto-Oncogene Proteins metabolism
Proto-Oncogene Proteins c-rel
HIV Long Terminal Repeat
NF-kappa B metabolism
Proto-Oncogene Proteins c-fos metabolism
Proto-Oncogene Proteins c-jun metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0261-4189
- Volume :
- 12
- Issue :
- 10
- Database :
- MEDLINE
- Journal :
- The EMBO journal
- Publication Type :
- Academic Journal
- Accession number :
- 8404856
- Full Text :
- https://doi.org/10.1002/j.1460-2075.1993.tb06066.x