Back to Search
Start Over
Relationship between functional Na+ pumps and mitogenesis in cultured coronary artery smooth muscle cells.
- Source :
-
The American journal of physiology [Am J Physiol] 1993 Jan; Vol. 264 (1 Pt 1), pp. C169-78. - Publication Year :
- 1993
-
Abstract
- An increase in functional sarcolemmal Na(+)-K(+)-ATPase (Na+ pump) precedes proliferation in vascular smooth muscle cells (VSMCs) seeded in 10% fetal bovine serum (FBS), but its role in mitogenesis is unresolved. Enzymatically dispersed canine coronary artery VSMCs were seeded in FBS and studied through confluence. Before a shift in cell cycle (G1-->S, G2 + M) and appearance of the nonmuscle isoform of myosin (MHCnm), intracellular Na+ content (Na+i) and cell volume (CV) increased (day 0 through day 3). Na+ pump number ([3H]-ouabain binding) increased at day 4 followed by a decrease in Na+i and CV. When Na+ pumps were inhibited by the addition of ouabain to FBS, VSMCs were arrested in G1, and MHCnm was not upregulated. Na+i increased similarly to that in FBS but failed to correct to day 0 levels. Withdrawal of ouabain at day 4 in culture led to an increase in Na+ pump number, a decrease in Na+i, entry of cells into S and G2 + M, and upregulation of MHCnm. These data suggest that Na+i, phenotypic modulation, and entry of cells into the cell cycle are temporally related, with Na+ pump-mediated correction of increased Na+i as a key event in the VSMC mitogenic process.
- Subjects :
- Animals
Cations metabolism
Cell Count
Cells, Cultured
Coronary Vessels cytology
Dogs
Female
Flow Cytometry
Male
Mitogens blood
Muscle, Smooth, Vascular cytology
Myosins metabolism
Ouabain metabolism
Sodium-Potassium-Exchanging ATPase drug effects
Coronary Vessels metabolism
Mitogens pharmacology
Muscle, Smooth, Vascular metabolism
Sodium-Potassium-Exchanging ATPase physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0002-9513
- Volume :
- 264
- Issue :
- 1 Pt 1
- Database :
- MEDLINE
- Journal :
- The American journal of physiology
- Publication Type :
- Academic Journal
- Accession number :
- 8381588
- Full Text :
- https://doi.org/10.1152/ajpcell.1993.264.1.C169