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Evidence for a role for protein kinase C in the stimulation of protein synthesis by insulin in swiss 3T3 fibroblasts.

Authors :
Welsh GI
Proud CG
Source :
FEBS letters [FEBS Lett] 1993 Feb 01; Vol. 316 (3), pp. 241-6.
Publication Year :
1993

Abstract

We have examined the role of protein kinase C (PKC) in the stimulation of protein synthesis by insulin using two complementary approaches. In the first, fibroblasts were pretreated with phorbol esters to down-regulate PKC. In these cells, the effects of insulin and of phorbol esters on protein synthesis were completely abolished, although serum still elicited an effect approaching that seen in control cells. Secondly, we used newly developed inhibitors of PKC which, again, blocked the effects of insulin and phorbol esters without greatly reducing the response to serum. Thus PKC apparently plays an important role in the stimulation of translation by insulin.

Details

Language :
English
ISSN :
0014-5793
Volume :
316
Issue :
3
Database :
MEDLINE
Journal :
FEBS letters
Publication Type :
Academic Journal
Accession number :
8380779
Full Text :
https://doi.org/10.1016/0014-5793(93)81300-o