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Evidence for a role for protein kinase C in the stimulation of protein synthesis by insulin in swiss 3T3 fibroblasts.
- Source :
-
FEBS letters [FEBS Lett] 1993 Feb 01; Vol. 316 (3), pp. 241-6. - Publication Year :
- 1993
-
Abstract
- We have examined the role of protein kinase C (PKC) in the stimulation of protein synthesis by insulin using two complementary approaches. In the first, fibroblasts were pretreated with phorbol esters to down-regulate PKC. In these cells, the effects of insulin and of phorbol esters on protein synthesis were completely abolished, although serum still elicited an effect approaching that seen in control cells. Secondly, we used newly developed inhibitors of PKC which, again, blocked the effects of insulin and phorbol esters without greatly reducing the response to serum. Thus PKC apparently plays an important role in the stimulation of translation by insulin.
- Subjects :
- 3T3 Cells
Animals
Guanine Nucleotide Exchange Factors
In Vitro Techniques
Indoles pharmacology
Maleimides pharmacology
Mice
Phorbol 12,13-Dibutyrate pharmacology
Protein Kinase C antagonists & inhibitors
Protein-Tyrosine Kinases metabolism
Proteins metabolism
Receptor, Insulin metabolism
Insulin pharmacology
Protein Biosynthesis
Protein Kinase C physiology
Subjects
Details
- Language :
- English
- ISSN :
- 0014-5793
- Volume :
- 316
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- FEBS letters
- Publication Type :
- Academic Journal
- Accession number :
- 8380779
- Full Text :
- https://doi.org/10.1016/0014-5793(93)81300-o