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Chromosome rearrangements recovered following transformation of Neurospora crassa.
- Source :
-
Genetics [Genetics] 1993 Jul; Vol. 134 (3), pp. 729-36. - Publication Year :
- 1993
-
Abstract
- New chromosome rearrangements were found in 10% or more of mitotically stable transformants. This was shown for transformations involving a variety of different markers, vectors and recipient strains. Breakpoints were randomly distributed among the seven linkage groups. Controls using untransformed protoplasts of the same strains contained almost no rearrangements. A study of molecularly characterized Am+ transformants showed that rearrangements are frequent when multiple ectopic integration events have occurred. In contrast, rearrangements are absent or infrequent when only the resident locus is restored to am+ by a homologous event. Sequences of the transforming vector were genetically linked to breakpoints in 6 of 10 translocations that were examined using Southern hybridization or colony blots.
Details
- Language :
- English
- ISSN :
- 0016-6731
- Volume :
- 134
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Genetics
- Publication Type :
- Academic Journal
- Accession number :
- 8349106
- Full Text :
- https://doi.org/10.1093/genetics/134.3.729