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The gene encoding the late nonstructural 36K protein of vaccinia virus is essential for virus reproduction.
- Source :
-
Virus research [Virus Res] 1993 Jun; Vol. 28 (3), pp. 273-83. - Publication Year :
- 1993
-
Abstract
- Two genetic markers--the thymidine kinase gene of herpes simplex virus, and the beta-galactosidase gene of Escherichia coli--were incorporated into the 36K protein gene (IL1 gene according to the nomenclature of the Copenhagen strain of vaccinia virus; Goebel et al., 1990) from the HindIII-P DNA fragment of the LIVP strain (variant of Lister strain) of vaccinia virus (VV). After recombination of the obtained integration plasmid pVZ64-TK with the VV genome (tk-), it was found that the resultant TK+ viruses were unstable with respect to the Lac+ phenotype. On the basis of hybridization of DNA fragments of selected clones, a scheme for the formation of hybrid viruses is proposed, and an approach to a simple phenotypical discrimination between essential and non-essential genes for VV viability is described.
Details
- Language :
- English
- ISSN :
- 0168-1702
- Volume :
- 28
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Virus research
- Publication Type :
- Academic Journal
- Accession number :
- 8346670
- Full Text :
- https://doi.org/10.1016/0168-1702(93)90027-k