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Production of factors with B-cell growth and differentiation activities by peripheral blood mononuclear cells from patients with hypogammaglobulinemia.
- Source :
-
The Journal of allergy and clinical immunology [J Allergy Clin Immunol] 1994 Jan; Vol. 93 (1 Pt 1), pp. 126-35. - Publication Year :
- 1994
-
Abstract
- Background: The maturation of normal B lymphocytes proceeds through a growth phase and a differentiation phase. These two phases appear to be under the influence of mediators released by immune cells, B-cell growth factor(s), which induce proliferation of B cells; and B-cell differentiation factor(s), which induce B-cell differentiation.<br />Methods: We analyzed the ability of peripheral blood mononuclear cells from patients with hypogammaglobulinemia to produce B-cell growth factor and B-cell differentiation factor activity in comparison with normal peripheral blood mononuclear cells.<br />Results: Of 27 patients tested, 26 had normal production of B-cell growth factor activity. A quantitative but not absolute defect in B-cell growth factor production was demonstrable in one boy with hypogammaglobulinemia. Interleukin-2 and interleukin-4 levels, as determined antigenically in these supernatants, had a similar distribution pattern from patients' or from control peripheral blood mononuclear cells; that is, undetectable levels of interleukin-2 were produced by cells from 4 of 16 patients tested and from 4 of 13 control subjects, and undetectable levels of interleukin-4 produced by cells from 6 of 16 patients and 4 of 13 control subjects. B-cell differentiation factor activity was absent in only one child tested but present in all other patients. Two patients had quantitatively low secretion of B-cell differentiation factor, but all others were within normal range. The two patients with quantitatively depressed B-cell differentiation factor activity had normal levels of B-cell growth factor activity, interleukin-2, and interleukin-4 produced from their cells.<br />Conclusion: Peripheral blood mononuclear cells from the majority of patients with hypogammaglobulinemia appear to have the capacity to produce B-cell growth factors and B-cell differentiation factor activity in vitro.
- Subjects :
- Adolescent
Adult
Cell Differentiation physiology
Cell Division physiology
Child
Child, Preschool
Concanavalin A pharmacology
Growth Substances metabolism
Humans
Interleukin-2 biosynthesis
Interleukin-4 biosynthesis
Interleukin-6 metabolism
Male
Middle Aged
Reference Values
Agammaglobulinemia blood
B-Lymphocytes pathology
Growth Substances physiology
Interleukin-6 physiology
Monocytes metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0091-6749
- Volume :
- 93
- Issue :
- 1 Pt 1
- Database :
- MEDLINE
- Journal :
- The Journal of allergy and clinical immunology
- Publication Type :
- Academic Journal
- Accession number :
- 8308179
- Full Text :
- https://doi.org/10.1016/0091-6749(94)90241-0