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Molecular cloning, sequencing, and characterizing the bovine receptor for gonadotropin releasing hormone (GnRH).
- Source :
-
Domestic animal endocrinology [Domest Anim Endocrinol] 1993 Oct; Vol. 10 (4), pp. 335-42. - Publication Year :
- 1993
-
Abstract
- Receptors for gonadotropin releasing hormone (GnRH), located in the cell membranes of adeno-hypophysial gonadotropes, mediate the action of GnRH to stimulate the secretion of the gonadotropic hormones (LH and FSH). In the present studies, we have isolated a GnRH receptor cDNA from bovine pituitary, determined its primary structure, and studied the regulation of its gene expression. The cDNA is composed of 1326 nucleotides and encodes a protein containing 328 amino acids. The GnRH receptor of cattle, like that in humans and mice, is a seven transmembrane receptor and has structural characteristics homologous with the family of G protein-coupled receptors. It exhibits 91% identity at the amino acid level with the human and 86% identity with mouse and rat receptors. Northern blot analysis of the RNA from bovine pituitary, probed with 32P-labeled bovine GnRH receptor cDNA, revealed the presence of four different transcripts (5.0, 3.5, 2.5 and 1.5 kb) in the pituitary of which the 5.0 kb form was most abundant. Using the reverse transcriptase/PCR technique, we detected expression of GnRH receptor mRNA in the pituitary but not in any other extrapituitary tissues such as the hypothalamus, hippocampus, testis, corpus luteum, ovary (containing follicles), myoendometrium, adrenal, kidney, liver and spleen. Higher levels of GnRH receptor mRNA were found in the pituitaries of steers than in cohort bulls, suggesting regulation of GnRH receptor gene expression by testicular steroids.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
Blotting, Northern
Cell Line
Cell Membrane chemistry
Cloning, Molecular
DNA Primers chemistry
Female
Gonadotropin-Releasing Hormone antagonists & inhibitors
Gonadotropin-Releasing Hormone metabolism
Male
Molecular Sequence Data
Open Reading Frames
Pituitary Gland chemistry
Pituitary Gland ultrastructure
RNA, Messenger biosynthesis
Receptors, LHRH biosynthesis
Receptors, LHRH chemistry
Receptors, LHRH metabolism
Sequence Homology, Nucleic Acid
Transcription, Genetic
Transfection
Cattle genetics
Gene Expression Regulation
Receptors, LHRH genetics
Subjects
Details
- Language :
- English
- ISSN :
- 0739-7240
- Volume :
- 10
- Issue :
- 4
- Database :
- MEDLINE
- Journal :
- Domestic animal endocrinology
- Publication Type :
- Academic Journal
- Accession number :
- 8306635
- Full Text :
- https://doi.org/10.1016/0739-7240(93)90037-c