Back to Search
Start Over
zeta-COP, a subunit of coatomer, is required for COP-coated vesicle assembly.
- Source :
-
The Journal of cell biology [J Cell Biol] 1993 Dec; Vol. 123 (6 Pt 2), pp. 1727-34. - Publication Year :
- 1993
-
Abstract
- cDNA encoding the 20-kD subunit of coatomer, zeta-COP, predicts a protein of 177-amino acid residues, similar in sequence to AP17 and AP19, subunits of the clathrin adaptor complexes. Polyclonal antibody directed to zeta-COP blocks the binding of coatomer to Golgi membranes and prevents the assembly of COP-coated vesicles on Golgi cisternae. Unlike other coatomer subunits (beta-, beta'-, gamma-, and epsilon-COP), zeta-COP exists in both coatomer bound and free pools.
- Subjects :
- Amino Acid Sequence
Animals
Base Sequence
CHO Cells
Cattle
Chromatography, Gel
Cricetinae
Cytosol metabolism
DNA Primers
DNA, Complementary metabolism
GTP-Binding Proteins biosynthesis
Golgi Apparatus drug effects
Golgi Apparatus ultrastructure
Guanosine 5'-O-(3-Thiotriphosphate) pharmacology
Intracellular Membranes ultrastructure
Macromolecular Substances
Membrane Proteins biosynthesis
Molecular Sequence Data
Molecular Weight
Oligonucleotides, Antisense
Polymerase Chain Reaction
Restriction Mapping
rhoB GTP-Binding Protein
Brain metabolism
Carrier Proteins biosynthesis
Clathrin biosynthesis
GTP-Binding Proteins metabolism
Golgi Apparatus metabolism
Intracellular Membranes metabolism
Liver metabolism
Membrane Proteins metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 0021-9525
- Volume :
- 123
- Issue :
- 6 Pt 2
- Database :
- MEDLINE
- Journal :
- The Journal of cell biology
- Publication Type :
- Academic Journal
- Accession number :
- 8276893
- Full Text :
- https://doi.org/10.1083/jcb.123.6.1727